Activation of multiple genes by provirus integration in the Mlvi-4 locus in T-cell lymphomas induced by Moloney murine leukemia virus - PubMed (original) (raw)

Comparative Study

Activation of multiple genes by provirus integration in the Mlvi-4 locus in T-cell lymphomas induced by Moloney murine leukemia virus

P N Tsichlis et al. J Virol. 1990 May.

Abstract

Moloney murine leukemia virus-induced rat T-cell lymphomas harbor proviruses integrated near c-myc and near Mlvi-1/Mis-1/Pvt-1, another locus of common integration which maps 270 kilobases 3' of c-myc. In this report, we present the characterization of a new locus of common integration in Moloney murine leukemia virus-induced T-cell lymphomas (Mlvi-4) which maps 30 kilobases 3' of c-myc, between c-myc and Mlvi-1. The Mlvi-4 locus, whose chromosomal map location is conserved in rats, mice, and humans, is also the target of chromosomal rearrangements in a variety of animal and human tumors. Evidence presented elsewhere shows that provirus integration in Mlvi-4 enhances the expression of c-myc and Mlvi-1 by cis-acting mechanisms operating over long distances of genomic DNA. In this manuscript, we show that provirus integration in the Mlvi-4 locus activates, by promoter insertion, one additional gene which maps immediately 3' to the cluster of the Mlvi-4 proviruses and which is transcribed in the same orientation as c-myc, giving rise to 3- and 10-kilobase mRNA transcripts. The Mlvi-4 gene is also expressed in normal thymus and spleen at very low levels, giving rise to 3- and 5.5-kilobase messages. Although Mlvi-4 is expressed in normal thymus, it is not expressed in Moloney murine leukemia virus-induced T-cell lymphomas corresponding to several stages of T-cell differentiation, but lacking a provirus in this locus. This suggests that Mlvi-4 may be expressed only in a subpopulation of T cells. We conclude that provirus insertion in Mlvi-4 activates c-myc and two additional genes, Mlvi-1 and Mlvi-4, whose expression is restricted to, and may be developmentally regulated in, T cells. Since Mlvi-4 is the target of genetic changes in a great variety of human and animal neoplasms, these results are critical for our understanding of oncogenesis.

PubMed Disclaimer

Similar articles

• Activation of the Mlvi-1/mis1/pvt-1 locus in Moloney murine leukemia virus-induced T-cell lymphomas.
  Tsichlis PN, Shepherd BM, Bear SE. Tsichlis PN, et al. Proc Natl Acad Sci U S A. 1989 Jul;86(14):5487-91. doi: 10.1073/pnas.86.14.5487. Proc Natl Acad Sci U S A. 1989. PMID: 2748599 Free PMC article.
• Long-distance activation of the Myc protooncogene by provirus insertion in Mlvi-1 or Mlvi-4 in rat T-cell lymphomas.
  Lazo PA, Lee JS, Tsichlis PN. Lazo PA, et al. Proc Natl Acad Sci U S A. 1990 Jan;87(1):170-3. doi: 10.1073/pnas.87.1.170. Proc Natl Acad Sci U S A. 1990. PMID: 1688653 Free PMC article.
• Oncogenesis by Moloney murine leukemia virus.
  Tsichlis PN. Tsichlis PN. Anticancer Res. 1987 Mar-Apr;7(2):171-80. Anticancer Res. 1987. PMID: 3592629
• Analysing uncharted transcriptomes with SAGE.
  Velculescu VE, Vogelstein B, Kinzler KW. Velculescu VE, et al. Trends Genet. 2000 Oct;16(10):423-5. doi: 10.1016/s0168-9525(00)02114-4. Trends Genet. 2000. PMID: 11050322 Review.
• Chromatin and viral integration in immunity: The challenge of silencing non-self genes.
  Kabi M, Filion GJ. Kabi M, et al. Trends Immunol. 2022 Jun;43(6):449-458. doi: 10.1016/j.it.2022.04.003. Epub 2022 Apr 27. Trends Immunol. 2022. PMID: 35490134 Review.

Cited by

• Mouse chromosome 15.
  Mock B, Neumann PE, Eppig JT, Huppi K. Mock B, et al. Mamm Genome. 1991;1 Spec No:S241-68. doi: 10.1007/BF00656496. Mamm Genome. 1991. PMID: 1799803 Review. No abstract available.
• Restricting activation-induced cytidine deaminase tumorigenic activity in B lymphocytes.
  Casellas R, Yamane A, Kovalchuk AL, Potter M. Casellas R, et al. Immunology. 2009 Mar;126(3):316-28. doi: 10.1111/j.1365-2567.2008.03050.x. Immunology. 2009. PMID: 19302140 Free PMC article. Review.
• Comparative map for mice and humans.
  Nadeau JH, Davisson MT, Doolittle DP, Grant P, Hillyard AL, Kosowsky M, Roderick TH. Nadeau JH, et al. Mamm Genome. 1991;1 Spec No:S461-515. doi: 10.1007/BF00656504. Mamm Genome. 1991. PMID: 1799811 Review. No abstract available.
• Twenty-seven nonoverlapping zinc finger cDNAs from human T cells map to nine different chromosomes with apparent clustering.
  Huebner K, Druck T, Croce CM, Thiesen HJ. Huebner K, et al. Am J Hum Genet. 1991 Apr;48(4):726-40. Am J Hum Genet. 1991. PMID: 2014798 Free PMC article.
• Multi-color RGB marking enables clonality assessment of liver tumors in a murine xenograft model.
  Thomaschewski M, Riecken K, Unrau L, Volz T, Cornils K, Ittrich H, Heim D, Wege H, Akgün E, Lütgehetmann M, Dieckhoff J, Köpke M, Dandri M, Benten D, Fehse B. Thomaschewski M, et al. Oncotarget. 2017 Dec 14;8(70):115582-115595. doi: 10.18632/oncotarget.23312. eCollection 2017 Dec 29. Oncotarget. 2017. PMID: 29383183 Free PMC article.

References

1. 1. Mol Cell Biol. 1986 Feb;6(2):380-92 - PubMed
2. 1. Somatic Cell Genet. 1977 Mar;3(2):231-6 - PubMed
3. 1. Proc Natl Acad Sci U S A. 1989 Oct;86(19):7495-9 - PubMed
4. 1. Proc Natl Acad Sci U S A. 1989 Aug;86(15):5678-82 - PubMed
5. 1. Cell. 1985 Jul;41(3):719-26 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Molecular Biology Databases

  • Mouse Genome Informatics (MGI)