Expression of voltage-gated K+ channels in insulin-producing cells. Analysis by polymerase chain reaction - PubMed (original) (raw)
Expression of voltage-gated K+ channels in insulin-producing cells. Analysis by polymerase chain reaction
C Betsholtz et al. FEBS Lett. 1990.
Free article
Abstract
We have used the polymerase chain reaction (PCR) with primers against the S5 and S6 regions of voltage-gated K+ channels to identify 8 different specific amplification products using poly(A)+ RNA isolated from islets of Langerhans from obese hyperglycemic (ob/ob) mice and from the two insulin-producing cell lines HIT T15 and RINm5F. Sequence analysis suggests that they derive from mRNAs coding for a family of voltage-gated K+ channels; 5 of these have been recently identified in mammalian brain and 3 are novel. These hybridize in classes to different mRNAs which distribute differently to a number of tissues and cell lines including insulin-producing cells.
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