Atomic resolution structures in nuclear transport - PubMed (original) (raw)

Atomic resolution structures in nuclear transport

Katherine E Süel et al. Methods. 2006 Aug.

Abstract

There are currently at least 53 structures of components of nuclear transport in the Protein Databank. In addition to providing critical insights into molecular mechanisms of nuclear transport, these atomic resolution structures provide a large body of information that could guide biochemical and cell biological analyses involving nuclear transport proteins. This paper catalogs 53 crystal and NMR structures of nuclear transport proteins, with the emphasis on providing information useful for mutagenesis and overexpression of recombinant proteins.

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Figures

Fig. 1

Fig. 1

Structures of Kapβ1/Impβ complexes. Kapβ1 proteins are shown as red ribbons. Substrates IBB (blue) and SREBP2 (orange) are drawn as cylinders and ribbons, PTHrp as a yellow ribbon. Ran is shown in green and nucleoporin peptides in blue (Nsp1p and the GLFG peptide drawn as stick figure and nup1p as cylinder/ribbon). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this paper.)

Fig. 2

Fig. 2

Structures of Kapβ2/Transportin complexes, Cse1p complexes and a Crm1 fragment. In the two Kapβ2 complexes, both Kapβ2 (red) and Ran (green) are shown as cylinders/ribbons and substrate M9NLS is shown as a cyan ribbon. Cse1p and Crm1 are both shown as purple cylinders/ribbons, and Ran (green) and Kapα (yellow) in the Cse1p-Ran-Kapα complex as ribbons. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this paper.)

Fig. 3

Fig. 3

Structures of mouse Kapα and yeast Kap60p complexes. Mouse Kapαs are all drawn as green ribbons and yeast Kap60p as blue ribbons. With the exception of Nup50p (red ribbon), all other ligands are shown as stick figures. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this paper.)

Fig. 4

Fig. 4

Structures of complexes involving Ran, NTF2, RanGAP and RanGEF. All 11 structures are shown as ribbon diagrams, with Ran in green, RanGAP in magenta, RCC1 RanGEF in blue, NTF2 in red, and RanBD1 or RanBP1-domain in gold (RanGppNHp-RBD1; 1RRP) and grey (RanGppNHp-RanBD1-RanGAP; 1K5D). RanGAP ligands, UBC9 is in aquamarine, SUMO is light brown and a fragment of Nup358 is in light green.

Fig. 5

Fig. 5

Structures of mRNA export factor TAP. TAP, Mex67p, p15 and MTR2p are all drawn as ribbons, and FG nucleoporin peptides are shown as stick figures. Domains of TAP and its yeast homolog Mex67p are in purple, p15 and its homolog Mtr2p are in red, and nucleoporin peptides in light green. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this paper.)

Fig. 6

Fig. 6

Structures of nucleoporin domains.

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