Endocarditis caused by extended-spectrum-beta-lactamase-producing Klebsiella pneumoniae: emergence of resistance to ciprofloxacin and piperacillin-tazobactam during treatment despite initial susceptibility - PubMed (original) (raw)

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Endocarditis caused by extended-spectrum-beta-lactamase-producing Klebsiella pneumoniae: emergence of resistance to ciprofloxacin and piperacillin-tazobactam during treatment despite initial susceptibility

Oren Zimhony et al. Antimicrob Agents Chemother. 2006 Sep.

Abstract

Three episodes of bacteremia occurred in the course of prosthetic valve endocarditis caused by an extended-spectrum-beta-lactamase (ESBL)-producing Klebsiella pneumoniae strain. The second isolate developed resistance to ciprofloxacin and the third isolate to piperacillin-tazobactam (PIP-TZ) following sequential therapy with each agent. The first isolate was resistant to PIP-TZ only at high inocula, the second isolate acquired increased transcription of the acrA gene, and the third isolate became resistant to PIP-TZ due to loss of beta-lactamase inhibition by TZ. We question if and how PIP-TZ susceptibility should be reported for ESBL-producing Enterobacteriaceae.

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Figures

FIG. 1.

FIG. 1.

Clinical course of treatment, including documentation of bacteremia and antimicrobial regimens used, for the patient described in the text. Cefepime was given at a dose of 2 g twice a day, ciprofloxacin at 400 mg twice a day, piperacillin-tazobactam at 4.5 g three times a day, amikacin at 500 mg twice a day, and meropenem at 1 g three times a day.

FIG. 2.

FIG. 2.

Determination of β-lactamase activity in cell-free crude extract by use of nitrocefin hydrolysis with and without tazobactam. β-Lactamase activity is expressed as units per milligram protein per minute.

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