Syk and Slp-76 mutant mice reveal a cell-autonomous hematopoietic cell contribution to vascular development - PubMed (original) (raw)

doi: 10.1016/j.devcel.2006.07.007.

Chris Hibbard, Shawn Sweeney, Farhad Abtahian, Natalie Bezman, Gina Clemens, Jonathan S Maltzman, Lan Cheng, Feiyan Liu, Martin Turner, Victor Tybulewicz, Gary A Koretzky, Mark L Kahn

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Syk and Slp-76 mutant mice reveal a cell-autonomous hematopoietic cell contribution to vascular development

Eric Sebzda et al. Dev Cell. 2006 Sep.

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Abstract

Developmental studies support a common origin for blood and endothelial cells, while studies of adult angiogenic responses suggest that the hematopoietic system can be a source of endothelial cells later in life. Whether hematopoietic tissue is a source of endothelial cells during normal vascular development is unknown. Mouse embryos lacking the signaling proteins Syk and Slp-76 develop abnormal blood-lymphatic endothelial connections. Here we demonstrate that expression of GFPSlp-76 in a subset of hematopoietic cells rescues this phenotype, and that deficient cells confer focal vascular phenotypes in chimeric embryos consistent with a cell-autonomous mechanism. Endogenous Syk and Slp-76, as well as transgenic GFPSlp-76, are expressed in circulating cells previously proposed to be endothelial precursors, supporting a causal role for these cells. These studies provide genetic evidence for hematopoietic contribution to vascular development and suggest that hematopoietic tissue can provide a source of vascular endothelial progenitor cells throughout life.

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