Structure of the 70S ribosome complexed with mRNA and tRNA - PubMed (original) (raw)

. 2006 Sep 29;313(5795):1935-42.

doi: 10.1126/science.1131127. Epub 2006 Sep 7.

Affiliations

• PMID: 16959973
• DOI: 10.1126/science.1131127

Structure of the 70S ribosome complexed with mRNA and tRNA

Maria Selmer et al. Science. 2006.

Abstract

The crystal structure of the bacterial 70S ribosome refined to 2.8 angstrom resolution reveals atomic details of its interactions with messenger RNA (mRNA) and transfer RNA (tRNA). A metal ion stabilizes a kink in the mRNA that demarcates the boundary between A and P sites, which is potentially important to prevent slippage of mRNA. Metal ions also stabilize the intersubunit interface. The interactions of E-site tRNA with the 50S subunit have both similarities and differences compared to those in the archaeal ribosome. The structure also rationalizes much biochemical and genetic data on translation.

PubMed Disclaimer

Similar articles

• Crystal structure of the ribosome at 5.5 A resolution.
  Yusupov MM, Yusupova GZ, Baucom A, Lieberman K, Earnest TN, Cate JH, Noller HF. Yusupov MM, et al. Science. 2001 May 4;292(5518):883-96. doi: 10.1126/science.1060089. Epub 2001 Mar 29. Science. 2001. PMID: 11283358
• X-ray crystal structures of 70S ribosome functional complexes.
  Cate JH, Yusupov MM, Yusupova GZ, Earnest TN, Noller HF. Cate JH, et al. Science. 1999 Sep 24;285(5436):2095-104. doi: 10.1126/science.285.5436.2095. Science. 1999. PMID: 10497122
• Ribosome structure. The ribosome in action.
  Dahlberg AE. Dahlberg AE. Science. 2001 May 4;292(5518):868-9. doi: 10.1126/science.1061513. Science. 2001. PMID: 11341282 No abstract available.
• The social life of ribosomal proteins.
  Brodersen DE, Nissen P. Brodersen DE, et al. FEBS J. 2005 May;272(9):2098-108. doi: 10.1111/j.1742-4658.2005.04651.x. FEBS J. 2005. PMID: 15853795 Review.
• High-resolution structures of large ribosomal subunits from mesophilic eubacteria and halophilic archaea at various functional States.
  Yonath A. Yonath A. Curr Protein Pept Sci. 2002 Feb;3(1):67-78. doi: 10.2174/1389203023380828. Curr Protein Pept Sci. 2002. PMID: 12370012 Review.

Cited by

• Non-coding RNA: a new frontier in regulatory biology.
  Fu XD. Fu XD. Natl Sci Rev. 2014 Jun 1;1(2):190-204. doi: 10.1093/nsr/nwu008. Natl Sci Rev. 2014. PMID: 25821635 Free PMC article.
• Ribosome•RelA structures reveal the mechanism of stringent response activation.
  Loveland AB, Bah E, Madireddy R, Zhang Y, Brilot AF, Grigorieff N, Korostelev AA. Loveland AB, et al. Elife. 2016 Jul 19;5:e17029. doi: 10.7554/eLife.17029. Elife. 2016. PMID: 27434674 Free PMC article.
• The impact of aminoglycosides on the dynamics of translation elongation.
  Tsai A, Uemura S, Johansson M, Puglisi EV, Marshall RA, Aitken CE, Korlach J, Ehrenberg M, Puglisi JD. Tsai A, et al. Cell Rep. 2013 Feb 21;3(2):497-508. doi: 10.1016/j.celrep.2013.01.027. Epub 2013 Feb 14. Cell Rep. 2013. PMID: 23416053 Free PMC article.
• Distinct tRNA Accommodation Intermediates Observed on the Ribosome with the Antibiotics Hygromycin A and A201A.
  Polikanov YS, Starosta AL, Juette MF, Altman RB, Terry DS, Lu W, Burnett BJ, Dinos G, Reynolds KA, Blanchard SC, Steitz TA, Wilson DN. Polikanov YS, et al. Mol Cell. 2015 Jun 4;58(5):832-44. doi: 10.1016/j.molcel.2015.04.014. Epub 2015 May 28. Mol Cell. 2015. PMID: 26028538 Free PMC article.
• An integrated, structure- and energy-based view of the genetic code.
  Grosjean H, Westhof E. Grosjean H, et al. Nucleic Acids Res. 2016 Sep 30;44(17):8020-40. doi: 10.1093/nar/gkw608. Epub 2016 Jul 22. Nucleic Acids Res. 2016. PMID: 27448410 Free PMC article. Review.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Ovid Technologies, Inc.

• Other Literature Sources

  • H1 Connect
  • The Lens - Patent Citations Database