Homing endonuclease I-CreI derivatives with novel DNA target specificities - PubMed (original) (raw)
Homing endonuclease I-CreI derivatives with novel DNA target specificities
Laura E Rosen et al. Nucleic Acids Res. 2006.
Abstract
Homing endonucleases are highly specific enzymes, capable of recognizing and cleaving unique DNA sequences in complex genomes. Since such DNA cleavage events can result in targeted allele-inactivation and/or allele-replacement in vivo, the ability to engineer homing endonucleases matched to specific DNA sequences of interest would enable powerful and precise genome manipulations. We have taken a step-wise genetic approach in analyzing individual homing endonuclease I-CreI protein/DNA contacts, and describe here novel interactions at four distinct target site positions. Crystal structures of two mutant endonucleases reveal the molecular interactions responsible for their altered DNA target specificities. We also combine novel contacts to create an endonuclease with the predicted target specificity. These studies provide important insights into engineering homing endonucleases with novel target specificities, as well as into the evolution of DNA recognition by this fascinating family of proteins.
Figures
Figure 1
I-CreI/DNA contacts. Direct protein/DNA hydrogen bonds are red; water molecules and water-mediated hydrogen bonds are blue. Palindromic bases are green; non-palindromic are white. Scissile phosphate groups are indicated with black dots.
Figure 2
In vivo endonuclease assays. Colony phenotypes resulting from transforming indicated I-CreI derivatives into cells harboring the indicated target site mutants (13). Increased proportion of white cells within colonies indicates increased site affinity. The Q26C/Y66R mutant has been described previously (14).
Figure 3
Relative cleavage of cognate and non-cognate targets. Wild-type (left) and Q44V (right) endonucleases were exposed to linearized plasmids containing wild-type and A:T ±4 homing sites. The left lanes in each gel (upper) indicate the enzyme concentration required for complete digestion of each substrate. Serial 2-fold dilutions were performed, and the relative enzyme concentrations required for 50% cleavage of each substrate determined (lower).
Figure 4
Structures and interactions of cognate pairs at residue 44 and base pair ±4 and residue 28 and base pair ±7. Protein/DNA contacts between wild-type I-CreI and its binding site are shown in (A and C). Contacts between Q44V and K28R and their cognate sites are shown in (B and D), respectively. The sequences of wild-type I-CreI target sequence and the alternate target sequences are shown below their corresponding structures. Dashed green lines represent hydrogen bonding and dashed magenta lines represent van der Waals interactions.
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