Ultra-high resolution array painting facilitates breakpoint sequencing - PubMed (original) (raw)
Case Reports
doi: 10.1136/jmg.2006.044909. Epub 2006 Sep 13.
D Kalaitzopoulos, D C Burford, E Prigmore, R R Selzer, B L Ng, N S W Matthews, K M Porter, R Curley, S J Lindsay, J Baptista, T A Richmond, N P Carter
Affiliations
- PMID: 16971479
- PMCID: PMC2597908
- DOI: 10.1136/jmg.2006.044909
Case Reports
Ultra-high resolution array painting facilitates breakpoint sequencing
S M Gribble et al. J Med Genet. 2007 Jan.
Abstract
Objective: To describe a considerably advanced method of array painting, which allows the rapid, ultra-high resolution mapping of translocation breakpoints such that rearrangement junction fragments can be amplified directly and sequenced.
Method: Ultra-high resolution array painting involves the hybridisation of probes generated by the amplification of small numbers of flow-sorted derivative chromosomes to oligonucleotide arrays designed to tile breakpoint regions at extremely high resolution.
Results and discussion: How ultra-high resolution array painting of four balanced translocation cases rapidly and efficiently maps breakpoints to a point where junction fragments can be amplified easily and sequenced is demonstrated. With this new development, breakpoints can be mapped using just two array experiments: the first using whole-genome array painting to tiling resolution large insert clone arrays, the second using ultra-high-resolution oligonucleotide arrays targeted to the breakpoint regions. In this way, breakpoints can be mapped and then sequenced in a few weeks.
Conflict of interest statement
Competing interests: None declared.
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