Hepatocyte-derived fibrinogen-related protein-1 is associated with the fibrin matrix of a plasma clot - PubMed (original) (raw)

. 2006 Nov 10;350(1):191-4.

doi: 10.1016/j.bbrc.2006.09.018. Epub 2006 Sep 15.

Affiliations

• PMID: 16996032
• DOI: 10.1016/j.bbrc.2006.09.018

Hepatocyte-derived fibrinogen-related protein-1 is associated with the fibrin matrix of a plasma clot

D C Rijken et al. Biochem Biophys Res Commun. 2006.

Abstract

In order to study the multiple functions of fibrinogen and fibrin, we are investigating which proteins bind to the fibrin matrix of a plasma clot by using a proteomic approach. Extracts from washed plasma clots were analysed by 2-D gel electrophoresis. A relatively abundant spot was identified as hepatocyte-derived fibrinogen-related protein-1 (HFREP-1) by MALDI-TOF analysis, molecular mass (34 kDa), iso-electric point (pI 5.5) as well as by Western blot analysis. HFREP-1 in plasma almost completely bound to the fibrin matrix during clot formation. Several purified fibrinogen preparations proved to be contaminated with HFREP-1. It is concluded that HFREP-1 (also named hepassocin), a protein with liver cell growth regulatory properties, occurs in plasma and strongly associates with fibrin and possibly fibrinogen.

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