Metagenomic analysis of two enhanced biological phosphorus removal (EBPR) sludge communities - PubMed (original) (raw)

. 2006 Oct;24(10):1263-9.

doi: 10.1038/nbt1247. Epub 2006 Sep 24.

Natalia Ivanova, Victor Kunin, Falk Warnecke, Kerrie W Barry, Alice C McHardy, Christine Yeates, Shaomei He, Asaf A Salamov, Ernest Szeto, Eileen Dalin, Nik H Putnam, Harris J Shapiro, Jasmyn L Pangilinan, Isidore Rigoutsos, Nikos C Kyrpides, Linda Louise Blackall, Katherine D McMahon, Philip Hugenholtz

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• PMID: 16998472
• DOI: 10.1038/nbt1247

Free article

Metagenomic analysis of two enhanced biological phosphorus removal (EBPR) sludge communities

Héctor García Martín et al. Nat Biotechnol. 2006 Oct.

Free article

Abstract

Enhanced biological phosphorus removal (EBPR) is one of the best-studied microbially mediated industrial processes because of its ecological and economic relevance. Despite this, it is not well understood at the metabolic level. Here we present a metagenomic analysis of two lab-scale EBPR sludges dominated by the uncultured bacterium, "Candidatus Accumulibacter phosphatis." The analysis sheds light on several controversies in EBPR metabolic models and provides hypotheses explaining the dominance of A. phosphatis in this habitat, its lifestyle outside EBPR and probable cultivation requirements. Comparison of the same species from different EBPR sludges highlights recent evolutionary dynamics in the A. phosphatis genome that could be linked to mechanisms for environmental adaptation. In spite of an apparent lack of phylogenetic overlap in the flanking communities of the two sludges studied, common functional themes were found, at least one of them complementary to the inferred metabolism of the dominant organism. The present study provides a much needed blueprint for a systems-level understanding of EBPR and illustrates that metagenomics enables detailed, often novel, insights into even well-studied biological systems.

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Comment in

• Wastewater genomics.
  Mino T, Satoh H. Mino T, et al. Nat Biotechnol. 2006 Oct;24(10):1229-30. doi: 10.1038/nbt1006-1229. Nat Biotechnol. 2006. PMID: 17033661 No abstract available.

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