Phylogenetic distribution of branched RNA-linked multicopy single-stranded DNA among natural isolates of Escherichia coli - PubMed (original) (raw)
Phylogenetic distribution of branched RNA-linked multicopy single-stranded DNA among natural isolates of Escherichia coli
P J Herzer et al. J Bacteriol. 1990 Nov.
Abstract
Multicopy single-stranded DNA (msDNA), a branched DNA-RNA molecule, has been shown in Escherichia coli B and clinical strain Cl-1 to be synthesized by reverse transcriptase. We report that 13% of the strains of the ECOR collection, a sample of 72 E. coli isolates representing the breadth of genetic variation of the species, produce msDNA. Three of the four major subspecific groups include msDNA-producing strains. Screening of 25 isolates that are genetically related to msDNA-producing clinical strains uncovered 22 additional msDNA-producing strains. A phylogenetic tree based on allelic variation detected electrophoretically at 20 enzyme-encoding loci revealed two major clusters and several deep branches composed of strains that synthesize msDNA. Although E. coli K-12 does not harbor msDNA, other closely related strains of the K-12 family do. The results support the hypothesis that msDNA-synthesizing systems, including reverse transcriptase genes, were acquired recently and independently in different lineages of E. coli.
References
- J Bacteriol. 1984 Feb;157(2):690-3 - PubMed
- J Gen Microbiol. 1983 Sep;129(9):2715-26 - PubMed
- Proc Natl Acad Sci U S A. 1983 Mar;80(6):1751-5 - PubMed
- Anal Biochem. 1987 Apr;162(1):156-9 - PubMed
- Infect Immun. 1988 Sep;56(9):2467-73 - PubMed
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