Short mucin 6 alleles are associated with H pylori infection - PubMed (original) (raw)

Short mucin 6 alleles are associated with H pylori infection

Thai V Nguyen et al. World J Gastroenterol. 2006.

Abstract

Aim: To investigate the relationship between mucin 6 (MUC6) VNTR length and H pylori infection.

Methods: Blood samples were collected from patients visiting the Can Tho General Hospital for upper gastrointestinal endoscopy. DNA was isolated from whole blood, the repeated section was cut out using a restriction enzyme (Pvu II) and the length of the allele fragments was determined by Southern blotting. H pylori infection was diagnosed by (14)C urea breath test. For analysis, MUC6 allele fragment length was dichotomized as being either long (> 13.5 kbp) or short (< or = 13.5 kbp) and patients were classified according to genotype [long-long (LL), long-short (LS), short-short (SS)].

Results: 160 patients were studied (mean age 43 years, 36% were males, 58% H pylori positive). MUC6 Pvu II-restricted allele fragment lengths ranged from 7 to 19 kbp. Of the patients with the LL, LS, SS MUC6 genotype, 43% (24/56), 57% (25/58) and 76% (11/46) were infected with H pylori, respectively (P = 0.003).

Conclusion: Short MUC6 alleles are associated with H pylori infection.

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Figures

Figure 1

Figure 1

Distribution of MUC6 allele fragment lengths after _Pvu_II restriction enzyme digestion.

Figure 2

Figure 2

Percentage of H pylori infected patients according to MUC6 genotype.

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References

    1. Kuipers EJ. Helicobacter pylori and the risk and management of associated diseases: gastritis, ulcer disease, atrophic gastritis and gastric cancer. Aliment Pharmacol Ther. 1997;11 Suppl 1:71–88. - PubMed
    1. Uemura N, Okamoto S, Yamamoto S, Matsumura N, Yamaguchi S, Yamakido M, Taniyama K, Sasaki N, Schlemper RJ. Helicobacter pylori infection and the development of gastric cancer. N Engl J Med. 2001;345:784–789. - PubMed
    1. Brown LM. Helicobacter pylori: epidemiology and routes of transmission. Epidemiol Rev. 2000;22:283–297. - PubMed
    1. Ota H, Nakayama J, Momose M, Hayama M, Akamatsu T, Katsuyama T, Graham DY, Genta RM. Helicobacter pylori infection produces reversible glycosylation changes to gastric mucins. Virchows Arch. 1998;433:419–426. - PubMed
    1. Van Klinken BJ, Dekker J, Büller HA, de Bolòs C, Einerhand AW. Biosynthesis of mucins (MUC2-6) along the longitudinal axis of the human gastrointestinal tract. Am J Physiol. 1997;273:G296–G302. - PubMed

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