Genetic variation in Opisthorchis viverrini (Trematoda: Opisthorchiidae) from northeast Thailand and Laos PDR based on random amplified polymorphic DNA analyses - PubMed (original) (raw)

Genetic variation in Opisthorchis viverrini (Trematoda: Opisthorchiidae) from northeast Thailand and Laos PDR based on random amplified polymorphic DNA analyses

Paiboon Sithithaworn et al. Parasitol Res. 2007 Feb.

Abstract

Genetic variation in Opisthorchis viverrini adults originating from different locations in northeast Thailand and Laos, People's Democratic Republic (PDR), was examined using random amplified polymorphic DNA (RAPD) analyses. In an initial analysis, the genomic DNA of one fluke from each of ten localities was amplified using 15 random primers (10-mers); however, genetic variation among O. viverrini specimens was detected reliably for only four primers. A more detailed RAPD analysis using these four primers was conducted on ten individuals from nine localities. Considerable genetic variation was detected among O. viverrini from different geographical areas and among some individuals from the same collecting locality. Comparison of the RAPD profiles revealed that O. viverrini adults from Laos PDR were genetically distinct from those from northeast Thailand. The taxonomic significance of this finding needs to be explored in more detail. The RAPD markers established in the present study provide opportunities to examine the biology and epidemiology of O. viverrini and fish-borne trematodes within the region. Additionally, application of these markers in such studies could have important implications in relation to the prevalence of cholangiocarcinoma in different regions of Asia.

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Figures

Fig. 1

Fig. 1

RAPD profiles of O. viverrini from different localities in northeastern Thailand and Laos (1 Nam Pong, Khon Kaen; 2 Mahasarakham; 3 Phuvaing, Khon Kaen; 4 Nakon Phanom; 5 Vientiane, Laos PDR; 6 Chaiya Phum; 7 Ban Lerngpleuy, Khon Kaen; 8 Ban Sa-ard, Khon Kaen; 9 Ban Phai, Khon Kaen and 10 Kalasin) generated by primer B17 (5′-AGGGAACGAG-3′)

Fig. 2

Fig. 2

Phenogram depicting the genetic differences of individual O. viverrini adults from sites in northeast Thailand and Laos PDR (KLp Ban Lerngpleuy, Khon Kaen; KBp Ban Phai, Khon Kaen; KNp Nam Pong, Khon Kaen; Kpv Phuvaing, Khon Kaen; MS Mahasarakham; NP Nakon Phanom; CP Chaiya Phum; KS Kalasin; VT Vientiane, Laos PDR) based on the RAPD profiles generated by four 10-mer primers (A2, A17, B5 and B17)

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