Direct measurement of torque in an optical trap and its application to double-strand DNA - PubMed (original) (raw)
Review
. 2006 Aug 4;97(5):058301.
doi: 10.1103/PhysRevLett.97.058301. Epub 2006 Aug 1.
Affiliations
- PMID: 17026144
- DOI: 10.1103/PhysRevLett.97.058301
Review
Direct measurement of torque in an optical trap and its application to double-strand DNA
László Oroszi et al. Phys Rev Lett. 2006.
Abstract
We present a method that offers the possibility to directly apply and measure torque on particles in an optical trap. It can be used to rotationally manipulate biopolymers attached to appropriate particles. A flat object is trapped and oriented in the focus of a linearly polarized laser light. The direction and power of the orientational trap are controlled by the polarization state of the light. As a demonstration of the capabilities of the method, we examined the torsional stiffness of dsDNA (lambda-DNA) in its linear torsional regime by directly measuring the torque generated by the molecule.
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