Junctional sequences influence the specificity of gamma/delta T cell receptors - PubMed (original) (raw)

Junctional sequences influence the specificity of gamma/delta T cell receptors

B L Rellahan et al. J Exp Med. 1991.

Abstract

T lymphocytes bearing the gamma/delta T cell receptor (TCR-gamma/delta) express a limited number of germline variable gene segments, generating receptor sequence diversity primarily through junctional mechanisms. To examine the role of V(D)J junctional sequences in antigen recognition by TCR-gamma/delta, we derived an alloreactive murine TCR-gamma/delta+ T cell line, LKD1, specific for the I-Ad class II major histocompatibility complex (MHC) molecule, and compared its receptor with that expressed by a previously characterized class II MHC alloreactive T cell line, LBK5, specific for I-Ek,b,s Ia molecules. Both LKD1 and LBK5 express receptors encoded by rearranged V gamma 1.2J gamma 2 and V delta 5D delta 2J delta 1 gene elements, differing in sequence only in the V(D)J junctional regions of the gamma and delta genes. These results demonstrate that junctionally encoded sequences corresponding to the putative third complementarity determining region can influence the antigen specificity of TCR-gamma/delta.

PubMed Disclaimer

Similar articles

• Structure and specificity of T cell receptor gamma/delta on major histocompatibility complex antigen-specific CD3+, CD4-, CD8- T lymphocytes.
  Bluestone JA, Cron RQ, Cotterman M, Houlden BA, Matis LA. Bluestone JA, et al. J Exp Med. 1988 Nov 1;168(5):1899-916. doi: 10.1084/jem.168.5.1899. J Exp Med. 1988. PMID: 2846743 Free PMC article.
• Structure and specificity of a class II MHC alloreactive gamma delta T cell receptor heterodimer.
  Matis LA, Fry AM, Cron RQ, Cotterman MM, Dick RF, Bluestone JA. Matis LA, et al. Science. 1989 Aug 18;245(4919):746-9. doi: 10.1126/science.2528206. Science. 1989. PMID: 2528206
• Diversity in junctional sequences associated with the common human V gamma 9 and V delta 2 gene segments in normal blood and lung compared with the limited diversity in a granulomatous disease.
  Tamura N, Holroyd KJ, Banks T, Kirby M, Okayama H, Crystal RG. Tamura N, et al. J Exp Med. 1990 Jul 1;172(1):169-81. doi: 10.1084/jem.172.1.169. J Exp Med. 1990. PMID: 2141626 Free PMC article.
• Specificity of gamma delta receptor bearing T cells.
  Matis LA, Bluestone JA. Matis LA, et al. Semin Immunol. 1991 Mar;3(2):75-80. Semin Immunol. 1991. PMID: 1716171 Review.
• Analysis of antigen specificity of human TCR gamma delta + T cells.
  Spits H, Yssel H, de Vries JE. Spits H, et al. Res Immunol. 1990 Sep;141(7):636-40. doi: 10.1016/0923-2494(90)90073-8. Res Immunol. 1990. PMID: 1710366 Review. No abstract available.

Cited by

• Specificity and selection of gamma-delta receptor-expressing T cells.
  Matis L. Matis L. Immunol Res. 1991;10(1):5-14. doi: 10.1007/BF02918163. Immunol Res. 1991. PMID: 1650806 Review. No abstract available.
• Predominant expansion of V gamma 9/V delta 2 T cells in a tularemia patient.
  Sumida T, Maeda T, Takahashi H, Yoshida S, Yonaha F, Sakamoto A, Tomioka H, Koike T, Yoshida S. Sumida T, et al. Infect Immun. 1992 Jun;60(6):2554-8. doi: 10.1128/iai.60.6.2554-2558.1992. Infect Immun. 1992. PMID: 1534075 Free PMC article.
• Peripheral selection of V delta 1+ cells with restricted T cell receptor delta gene junctional repertoire in the peripheral blood of healthy donors.
  Beldjord K, Beldjord C, Macintyre E, Even P, Sigaux F. Beldjord K, et al. J Exp Med. 1993 Jul 1;178(1):121-7. doi: 10.1084/jem.178.1.121. J Exp Med. 1993. PMID: 8391058 Free PMC article.
• The delta T cell receptor repertoire in human colon and peripheral blood is oligoclonal irrespective of V region usage.
  Holtmeier W, Chowers Y, Lumeng A, Morzycka-Wroblewska E, Kagnoff MF. Holtmeier W, et al. J Clin Invest. 1995 Aug;96(2):1108-17. doi: 10.1172/JCI118097. J Clin Invest. 1995. PMID: 7635946 Free PMC article.
• Exploring single-cell data with deep multitasking neural networks.
  Amodio M, van Dijk D, Srinivasan K, Chen WS, Mohsen H, Moon KR, Campbell A, Zhao Y, Wang X, Venkataswamy M, Desai A, Ravi V, Kumar P, Montgomery R, Wolf G, Krishnaswamy S. Amodio M, et al. Nat Methods. 2019 Nov;16(11):1139-1145. doi: 10.1038/s41592-019-0576-7. Epub 2019 Oct 7. Nat Methods. 2019. PMID: 31591579 Free PMC article.

References

1. 1. J Exp Med. 1988 Nov 1;168(5):1899-916 - PubMed
2. 1. Nature. 1988 Feb 18;331(6157):627-31 - PubMed
3. 1. J Immunol. 1989 Dec 1;143(11):3769-75 - PubMed
4. 1. Cell. 1988 Aug 12;54(4):473-84 - PubMed
5. 1. Science. 1989 Aug 18;245(4919):746-9 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • Ovid Technologies, Inc.
  • PubMed Central
  • Silverchair Information Systems

• Research Materials

  • NCI CPTC Antibody Characterization Program