Demonstration of the origin of human mast cells from CD34+ bone marrow progenitor cells - PubMed (original) (raw)
. 1991 Mar 1;146(5):1410-5.
Affiliations
- PMID: 1704394
Demonstration of the origin of human mast cells from CD34+ bone marrow progenitor cells
A S Kirshenbaum et al. J Immunol. 1991.
Abstract
It has been established that murine mast cells are derived from a pluripotent bone marrow stem cell. In humans, the corresponding pluripotent cell is included in the CD34+ bone marrow population. To determine whether human mast cells arise from CD34+ human progenitor cells, enriched CD34+ cells were cultured over agarose surfaces (interphase cultures) or cocultured with mouse 3T3 fibroblasts in the presence of recombinant human (rh) IL-3. The presence of both mast cells and basophils was determined using a variety of histochemical and immunohistologic techniques, including immunogold labeling for IgE receptors and mast cell tryptase. Mast cells and basophils continued to appear in cultures when T cell, B cell, macrophage, and eosinophil committed progenitor cells were removed, but were not seen in cultures from which CD34+ cells were removed. CD34+ cells layered over agarose in the presence of rhIL-3 were shown to give rise to cultures that contained mast cells (1 to 5%) and basophils (25 to 40%). Cultures supplemented with rhIL-4 showed no additional increase in mast cells or basophils. CD34+ cells cocultured with 3T3 fibroblasts in the presence of rhIL-3 gave rise to mast cells within the fibroblast monolayer, which by 6 wk comprised up to 46% of the monolayer. CD34-cells on 3T3 fibroblasts gave rise to few mast cells (2% of the monolayer). Mast cell granules from interphase cultures contained homogeneous electron-dense material. In contrast, mast cells within 3T3 monolayers at 6 wk contained a variety of granule morphologies, including scroll, mixed, reticular, dense core, or homogeneous patterns. We conclude that both human mast cells and basophils arise from CD34+ human progenitor cells.
Similar articles
- Effect of IL-3 and stem cell factor on the appearance of human basophils and mast cells from CD34+ pluripotent progenitor cells.
Kirshenbaum AS, Goff JP, Kessler SW, Mican JM, Zsebo KM, Metcalfe DD. Kirshenbaum AS, et al. J Immunol. 1992 Feb 1;148(3):772-7. J Immunol. 1992. PMID: 1370517 - Monocytes do not make mast cells when cultured in the presence of SCF. Characterization of the circulating mast cell progenitor as a c-kit+, CD34+, Ly-, CD14-, CD17-, colony-forming cell.
Agis H, Willheim M, Sperr WR, Wilfing A, Krömer E, Kabrna E, Spanblöchl E, Strobl H, Geissler K, Spittler A, Boltz-Nitulescu G, Majdic O, Lechner K, Valent P. Agis H, et al. J Immunol. 1993 Oct 15;151(8):4221-7. J Immunol. 1993. PMID: 7691941 - IL-3-dependent growth of basophil-like cells and mastlike cells from human bone marrow.
Kirshenbaum AS, Goff JP, Dreskin SC, Irani AM, Schwartz LB, Metcalfe DD. Kirshenbaum AS, et al. J Immunol. 1989 Apr 1;142(7):2424-9. J Immunol. 1989. PMID: 2647850 - Ultrastructural analysis of the development of human basophils and mast cells in vitro.
Dvorak AM, Ishizaka T. Dvorak AM, et al. Int J Clin Lab Res. 1995;25(1):7-24. doi: 10.1007/BF02592571. Int J Clin Lab Res. 1995. PMID: 7787214 Review. - Cytokines involved in growth and differentiation of human basophils and mast cells.
Valent P. Valent P. Exp Dermatol. 1995 Aug;4(4 Pt 2):255-9. doi: 10.1111/j.1600-0625.1995.tb00254.x. Exp Dermatol. 1995. PMID: 8528598 Review.
Cited by
- MRGPRX2-mediated mast cell activation by substance P from overloaded human tenocytes induces inflammatory and degenerative responses in tendons.
Mousavizadeh R, Waugh CM, McCormack RG, Cairns BE, Scott A. Mousavizadeh R, et al. Sci Rep. 2024 Jun 12;14(1):13540. doi: 10.1038/s41598-024-64222-1. Sci Rep. 2024. PMID: 38866832 Free PMC article. - Description and Characterization of Three-Dimensional Human Mast Cell Progenitor Spheroids In Vitro.
Praetzel R, Motaghed M, Fereydouni M, Ahani E, Kepley C. Praetzel R, et al. Cureus. 2024 Feb 6;16(2):e53708. doi: 10.7759/cureus.53708. eCollection 2024 Feb. Cureus. 2024. PMID: 38455803 Free PMC article. - Identification of an immunodominant IgE epitope of Der f 40, a novel allergen of Dermatophagoides farinae.
Cai ZL, Liu S, Li WY, Zhou ZW, Hu WZ, Chen JJ, Ji K. Cai ZL, et al. World Allergy Organ J. 2023 Aug 1;16(8):100804. doi: 10.1016/j.waojou.2023.100804. eCollection 2023 Aug. World Allergy Organ J. 2023. PMID: 37577028 Free PMC article. - Mast cell ontogeny: From fetal development to life-long health and disease.
Chia SL, Kapoor S, Carvalho C, Bajénoff M, Gentek R. Chia SL, et al. Immunol Rev. 2023 May;315(1):31-53. doi: 10.1111/imr.13191. Epub 2023 Feb 8. Immunol Rev. 2023. PMID: 36752151 Free PMC article. Review. - A Missense Variant in PLP2 in Holstein Cattle with X-Linked Congenital Mast Cell Tumor.
Jacinto JGP, Muscatello LV, Häfliger IM, Benazzi C, Bolcato M, Gentile A, Drögemüller C. Jacinto JGP, et al. Animals (Basel). 2022 Sep 7;12(18):2329. doi: 10.3390/ani12182329. Animals (Basel). 2022. PMID: 36139188 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Medical