Pax6 is regulated by Meis and Pbx homeoproteins during pancreatic development - PubMed (original) (raw)
. 2006 Dec 15;300(2):748-57.
doi: 10.1016/j.ydbio.2006.06.030. Epub 2006 Jun 27.
Affiliations
- PMID: 17049510
- DOI: 10.1016/j.ydbio.2006.06.030
Free article
Pax6 is regulated by Meis and Pbx homeoproteins during pancreatic development
Xin Zhang et al. Dev Biol. 2006.
Free article
Abstract
Pancreatic development depends on the transcription factor Pax6, which controls islet cell differentiation and hormone production. To understand the regulation of Pax6 pancreatic expression, we have identified a minimal Pax6 pancreatic enhancer and show that it contains a composite binding site for Meis and Pbx homeoproteins. We further show that Meis proteins are expressed during pancreatic development, and together with Pbx, are able to form a synergistic binding complex on the Pax6 pancreatic enhancer. When tested in transgenic mice, both the Meis and Pbx sites are essential for Pax6 pancreatic enhancer activity, and the composite site can be functionally replaced by a consensus Meis-Pbx sequence. In addition, analysis of Pbx1 and Pbx2 knockout mice demonstrates that, during pancreatic islet formation, Pax6 expression becomes dependent upon Pbx1 and Pbx2 function. As Meis homeoproteins have been previously demonstrated to regulate Pax6 expression during lens development, these results suggest a conserved mechanism of Pax6 regulation by Meis homeoproteins in two different organs.
Similar articles
- The functional role of the Meis/Prep-binding elements in Pax6 locus during pancreas and eye development.
Carbe C, Hertzler-Schaefer K, Zhang X. Carbe C, et al. Dev Biol. 2012 Mar 1;363(1):320-9. doi: 10.1016/j.ydbio.2011.12.038. Epub 2012 Jan 3. Dev Biol. 2012. PMID: 22240097 Free PMC article. - Expression of zebrafish pax6b in pancreas is regulated by two enhancers containing highly conserved cis-elements bound by PDX1, PBX and PREP factors.
Delporte FM, Pasque V, Devos N, Manfroid I, Voz ML, Motte P, Biemar F, Martial JA, Peers B. Delporte FM, et al. BMC Dev Biol. 2008 May 16;8:53. doi: 10.1186/1471-213X-8-53. BMC Dev Biol. 2008. PMID: 18485195 Free PMC article. - PAX6 and SOX2-dependent regulation of the Sox2 enhancer N-3 involved in embryonic visual system development.
Inoue M, Kamachi Y, Matsunami H, Imada K, Uchikawa M, Kondoh H. Inoue M, et al. Genes Cells. 2007 Sep;12(9):1049-61. doi: 10.1111/j.1365-2443.2007.01114.x. Genes Cells. 2007. PMID: 17825048 - Interplay of Pax6 and SOX2 in lens development as a paradigm of genetic switch mechanisms for cell differentiation.
Kondoh H, Uchikawa M, Kamachi Y. Kondoh H, et al. Int J Dev Biol. 2004;48(8-9):819-27. doi: 10.1387/ijdb.041868hk. Int J Dev Biol. 2004. PMID: 15558474 Review. - Glucagon gene expression in the endocrine pancreas: the role of the transcription factor Pax6 in α-cell differentiation, glucagon biosynthesis and secretion.
Gosmain Y, Cheyssac C, Heddad Masson M, Dibner C, Philippe J. Gosmain Y, et al. Diabetes Obes Metab. 2011 Oct;13 Suppl 1:31-8. doi: 10.1111/j.1463-1326.2011.01445.x. Diabetes Obes Metab. 2011. PMID: 21824254 Review.
Cited by
- The functional role of the Meis/Prep-binding elements in Pax6 locus during pancreas and eye development.
Carbe C, Hertzler-Schaefer K, Zhang X. Carbe C, et al. Dev Biol. 2012 Mar 1;363(1):320-9. doi: 10.1016/j.ydbio.2011.12.038. Epub 2012 Jan 3. Dev Biol. 2012. PMID: 22240097 Free PMC article. - Monoallelic variants resulting in substitutions of MAB21L1 Arg51 Cause Aniridia and microphthalmia.
Hall HN, Bengani H, Hufnagel RB, Damante G, Ansari M, Marsh JA, Grimes GR, Kriegsheim AV, Moore D, McKie L, Rahmat J, Mio C, Blyth M, Keng WT, Islam L, McEntargart M, Mannens MM, Heyningen VV, Rainger J, Brooks BP, FitzPatrick DR. Hall HN, et al. PLoS One. 2022 Nov 22;17(11):e0268149. doi: 10.1371/journal.pone.0268149. eCollection 2022. PLoS One. 2022. PMID: 36413568 Free PMC article. - Considerations for the use of Cre recombinase for conditional gene deletion in the mouse lens.
Lam PT, Padula SL, Hoang TV, Poth JE, Liu L, Liang C, LeFever AS, Wallace LM, Ashery-Padan R, Riggs PK, Shields JE, Shaham O, Rowan S, Brown NL, Glaser T, Robinson ML. Lam PT, et al. Hum Genomics. 2019 Feb 15;13(1):10. doi: 10.1186/s40246-019-0192-8. Hum Genomics. 2019. PMID: 30770771 Free PMC article. - Transcription factor PAX6 as a novel prognostic factor and putative tumour suppressor in non-small cell lung cancer.
Kiselev Y, Andersen S, Johannessen C, Fjukstad B, Standahl Olsen K, Stenvold H, Al-Saad S, Donnem T, Richardsen E, Bremnes RM, Rasmussen Busund LT. Kiselev Y, et al. Sci Rep. 2018 Mar 22;8(1):5059. doi: 10.1038/s41598-018-23417-z. Sci Rep. 2018. PMID: 29568088 Free PMC article. - Pbx/Meis deficiencies demonstrate multigenetic origins of congenital heart disease.
Stankunas K, Shang C, Twu KY, Kao SC, Jenkins NA, Copeland NG, Sanyal M, Selleri L, Cleary ML, Chang CP. Stankunas K, et al. Circ Res. 2008 Sep 26;103(7):702-9. doi: 10.1161/CIRCRESAHA.108.175489. Epub 2008 Aug 21. Circ Res. 2008. PMID: 18723445 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials