Humanized mice mount specific adaptive and innate immune responses to EBV and TSST-1 - PubMed (original) (raw)
. 2006 Nov;12(11):1316-22.
doi: 10.1038/nm1431. Epub 2006 Oct 22.
Affiliations
- PMID: 17057712
- DOI: 10.1038/nm1431
Humanized mice mount specific adaptive and innate immune responses to EBV and TSST-1
Michael W Melkus et al. Nat Med. 2006 Nov.
Abstract
Here we show that transplantation of autologous human hematopoietic fetal liver CD34+ cells into NOD/SCID mice previously implanted with human fetal thymic and liver tissues results in long-term, systemic human T-cell homeostasis. In addition, these mice show systemic repopulation with human B cells, monocytes and macrophages, and dendritic cells (DCs). T cells in these mice generate human major histocompatibility complex class I- and class II-restricted adaptive immune responses to Epstein-Barr virus (EBV) infection and are activated by human DCs to mount a potent T-cell immune response to superantigens. Administration of the superantigen toxic shock syndrome toxin 1 (TSST-1) results in the specific systemic expansion of human Vbeta2+ T cells, release of human proinflammatory cytokines and localized, specific activation and maturation of human CD11c+ dendritic cells. This represents the first demonstration of long-term systemic human T-cell reconstitution in vivo allowing for the manifestation of the differential response by human DCs to TSST-1.
Similar articles
- Human peripheral gammadelta T cells potentiate the early proinflammatory cytokine response to staphylococcal toxic shock syndrome toxin-1.
Kalyan S, Chow AW. Kalyan S, et al. J Infect Dis. 2004 May 15;189(10):1892-6. doi: 10.1086/383478. Epub 2004 Apr 26. J Infect Dis. 2004. PMID: 15122526 - Vbeta-restricted T cell adherence to endothelial cells: a mechanism for superantigen-dependent vascular injury.
Brogan PA, Shah V, Klein N, Dillon MJ. Brogan PA, et al. Arthritis Rheum. 2004 Feb;50(2):589-97. doi: 10.1002/art.20021. Arthritis Rheum. 2004. PMID: 14872503 - Streptococcus pyogenes: Insight into the function of the streptococcal superantigens.
Sriskandan S, Faulkner L, Hopkins P. Sriskandan S, et al. Int J Biochem Cell Biol. 2007;39(1):12-9. doi: 10.1016/j.biocel.2006.08.009. Epub 2006 Sep 1. Int J Biochem Cell Biol. 2007. PMID: 17029999 Review. - Innate Valpha14(+) natural killer T cells mature dendritic cells, leading to strong adaptive immunity.
Fujii S, Shimizu K, Hemmi H, Steinman RM. Fujii S, et al. Immunol Rev. 2007 Dec;220:183-98. doi: 10.1111/j.1600-065X.2007.00561.x. Immunol Rev. 2007. PMID: 17979847 Review.
Cited by
- Advancing Human Vaccine Development Using Humanized Mouse Models.
Han R, Su L, Cheng L. Han R, et al. Vaccines (Basel). 2024 Sep 4;12(9):1012. doi: 10.3390/vaccines12091012. Vaccines (Basel). 2024. PMID: 39340042 Free PMC article. Review. - HIV Persistence, Latency, and Cure Approaches: Where Are We Now?
Chou TC, Maggirwar NS, Marsden MD. Chou TC, et al. Viruses. 2024 Jul 19;16(7):1163. doi: 10.3390/v16071163. Viruses. 2024. PMID: 39066325 Free PMC article. Review. - HIV broadly neutralizing antibody escapability drives the therapeutic efficacy of vectored immunotherapy.
Galvez NMS, Cao Y, Nitido AD, Deal CE, Boutros CL, MacDonald SW, Albrecht YES, Lam EC, Sheehan ML, Parsons D, Lin AZ, Deymier MJ, Brady JM, Moon B, Bullock CB, Tanno S, Pegu A, Chen X, Liu C, Koup RA, Mascola JR, Vrbanac VD, Lingwood D, Balazs AB. Galvez NMS, et al. bioRxiv [Preprint]. 2024 Jul 13:2024.07.11.603156. doi: 10.1101/2024.07.11.603156. bioRxiv. 2024. PMID: 39026699 Free PMC article. Preprint. - Protocol to construct humanized mice with adult CD34+ hematopoietic stem and progenitor cells.
Yu CI, Maser R, Marches F, Banchereau J, Palucka K. Yu CI, et al. STAR Protoc. 2024 Sep 20;5(3):103155. doi: 10.1016/j.xpro.2024.103155. Epub 2024 Jun 26. STAR Protoc. 2024. PMID: 38935509 Free PMC article. - Humanized mouse model for vaccine evaluation: an overview.
Kaushik S, Kumari L, Deepak RK. Kaushik S, et al. Clin Exp Vaccine Res. 2024 Jan;13(1):10-20. doi: 10.7774/cevr.2024.13.1.10. Epub 2024 Jan 31. Clin Exp Vaccine Res. 2024. PMID: 38362371 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
- 5T32 AI005284/AI/NIAID NIH HHS/United States
- AI39416/AI/NIAID NIH HHS/United States
- CA82055/CA/NCI NIH HHS/United States
- HD011149/HD/NICHD NIH HHS/United States
- R37 AI028246/AI/NIAID NIH HHS/United States
- T32 AI07421/AI/NIAID NIH HHS/United States
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials