Induction of lymphangiogenesis in and around axillary lymph node metastases of patients with breast cancer - PubMed (original) (raw)

Comparative Study

. 2006 Nov 20;95(10):1362-6.

doi: 10.1038/sj.bjc.6603443. Epub 2006 Oct 31.

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Comparative Study

Induction of lymphangiogenesis in and around axillary lymph node metastases of patients with breast cancer

G G Van den Eynden et al. Br J Cancer. 2006.

Abstract

We studied the presence of lymphangiogenesis in lymph node (LN) metastases of breast cancer. Lymph vessels were present in 52 of 61 (85.2%) metastatically involved LNs vs 26 of 104 (25.0%) uninvolved LNs (P<0.001). Furthermore, median intra- and perinodal lymphatic endothelial cell proliferation fractions were higher in metastatically involved LNs (P<0.001). This is the first report demonstrating lymphangiogenesis in LN metastases of cancer in general and breast cancer in particular.

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Figures

Figure 1

Figure 1

Overview of the immunohistochemical stains used on serial sections of a metastatically uninvolved (perinodal fat: A–B; LN: C–F) and involved LN (G–L). In uninvolved LNs, high endothelial venules (*) show CD34 (C) and CD31 (D) reactivity. Littoral cells (+) are positive for CD31 (D) and Prox-1 (F). In metastatically involved LNs, podoplanin-positive LVs (red arrow) are demonstrated (G). Blood vessels (yellow arrow) are podoplanin negative (G). The LVs show CD34 (J), CD31 (K) and Prox-1 (I) reactivity. Although in perinodal fat (B) LVs are strongly positive for LYVE-1, the LVs in metastatically involved LNs are only faintly positive for LYVE-1 (H). Furthermore, a proliferating lymphatic endothelial cell is shown at the border of a LN metastasis (L) (LN: lymph node; LV: lymph vessel).

Figure 2

Figure 2

Comparison of intranodal (A) and perinodal (B) LECP% between metastatically involved and uninvolved axillary LNs in patients with LN-negative and LN-positive breast cancer. Both intra- (P<0.001) and perinodal (P<0.001) LECP% is significantly increased in metastatically involved LNs compared to uninvolved LNs (LECP%: lymphatic endothelial cell proliferation fraction; LN: lymph node; N0: lymph node negative; N+: lymph node positive).

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References

    1. Angeli V, Ginhoux F, Llodrà J, Quemeneur L, Frenette P, Skobe M, Jessberger R, Merad M, Randolph G (2006) B cell-driven lymphangiogenesis in inflamed lymph nodes enhances dendritic cell mobilization. Immunity 24: 203. - PubMed
    1. Beasley N, Prevo R, Banerji S, Leek R, Moore J, Van Trappen P, Cox G, Harris AL, Jackson DG (2002) Intratumoral lymphangiogenesis and lymph node metastasis in head and neck cancer. Cancer Res 62: 1315. - PubMed
    1. Dadras SS, Bertoncini J, Brown L, Muzikansky A, Jackson D, Ellwanger U, Garbe C, Mihm M, Detmar M (2003) Tumor lymphangiogenesis: a novel prognostic indicator for cutaneous melanoma metastasis and survival. Am J Pathol 162: 1951. - PMC - PubMed
    1. Evangelou E, Kyzas P, Trikalinos T (2005) Comparison of the diagnostic accuracy of lymphatic endothelium markers: Bayesian approach. Mod Pathol 18: 1490. - PubMed
    1. Guidi A, Berry D, Broadwater G, Perloff M, Norton L, Barcos MP, Hayes D (2000) Association of angiogenesis in lymph node metastases with outcome of breast cancer. J Natl Cancer Inst 92: 486. - PubMed

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