Effects of neonatal maternal separation on neurochemical and sensory response to colonic distension in a rat model of irritable bowel syndrome - PubMed (original) (raw)
. 2007 Mar;292(3):G849-56.
doi: 10.1152/ajpgi.00400.2006. Epub 2006 Nov 16.
Affiliations
- PMID: 17110521
- DOI: 10.1152/ajpgi.00400.2006
Free article
Effects of neonatal maternal separation on neurochemical and sensory response to colonic distension in a rat model of irritable bowel syndrome
Tian-Hua Ren et al. Am J Physiol Gastrointest Liver Physiol. 2007 Mar.
Free article
Abstract
Early life stress has been implicated as a risk factor for irritable bowel syndrome (IBS). We studied the effect of neonatal maternal separation on the visceromotor response and the expression of c-fos, 5-HT, and its receptors/transporters along the brain-gut axis in an animal model of IBS. Male neonatal Sprague-Dawley rats were randomly assigned to a 3-h daily maternal separation (MS) or nonhandling (NH) on postnatal days 2-21. Colorectal balloon distention (CRD) was performed for assessment of abdominal withdrawal reflex as a surrogate marker of visceral pain. Tissues from dorsal raphe nucleus in midbrain, lumbar-sacral cord, and distal colon were harvested for semiquantitative analysis of c-fos and 5-HT. The expression of 5-HT expression, 5-HT3 receptors, and 5-HT transporter were analyzed by RT-PCR. Pain threshold was significantly lower in MS than NH rats. The abdominal withdrawal reflex score in response to CRD in MS rats was significantly higher with distension pressures of 40, 60, and 80 mmHg. In MS rats, the number of c-fos-like immunoreactive nuclei at dorsal horn of lumbar-sacral spinal cord increased significantly after CRD. 5-HT content in the spinal cord of MS rats was significant higher. In the colon, both 5-HT-positive cell number and 5-HT content were comparable between MS and NH groups before CRD. Post-CRD only MS rats had significant increase in 5-HT content. Protein and mRNA expression levels of 5-HT3 receptors and 5-HT transporter were similar in MS and NH rats. Neonatal maternal separation stress predisposes rats to exaggerated neurochemical responses and visceral hyperalgesia in colon mimicking IBS.
Similar articles
- Neonatal maternal separation enhances central sensitivity to noxious colorectal distention in rat.
Chung EK, Zhang X, Li Z, Zhang H, Xu H, Bian Z. Chung EK, et al. Brain Res. 2007 Jun 11;1153:68-77. doi: 10.1016/j.brainres.2007.03.047. Epub 2007 Mar 23. Brain Res. 2007. PMID: 17434464 - Lactobacillus farciminis treatment attenuates stress-induced overexpression of Fos protein in spinal and supraspinal sites after colorectal distension in rats.
Ait-Belgnaoui A, Eutamene H, Houdeau E, Bueno L, Fioramonti J, Theodorou V. Ait-Belgnaoui A, et al. Neurogastroenterol Motil. 2009 May;21(5):567-73, e18-9. doi: 10.1111/j.1365-2982.2009.01280.x. Epub 2009 Feb 27. Neurogastroenterol Motil. 2009. PMID: 19309441 - [Role of serotonin in the pathophysiology of the irritable bowel syndrome].
Moskwa A, Boznańska P. Moskwa A, et al. Wiad Lek. 2007;60(7-8):371-6. Wiad Lek. 2007. PMID: 18175558 Review. Polish. - Behavioral and serotonergic consequences of decreasing or increasing hippocampus brain-derived neurotrophic factor protein levels in mice.
Deltheil T, Guiard BP, Cerdan J, David DJ, Tanaka KF, Repérant C, Guilloux JP, Coudoré F, Hen R, Gardier AM. Deltheil T, et al. Neuropharmacology. 2008 Nov;55(6):1006-14. doi: 10.1016/j.neuropharm.2008.08.001. Epub 2008 Aug 12. Neuropharmacology. 2008. PMID: 18761360 Review.
Cited by
- Effect of TongXie-YaoFang on Cl(-) and HCO3 (-) Transport in Diarrhea-Predominant Irritable Bowel Syndrome Rats.
Lu X, Zhang S, Yang C, Wang Z, Zhao L, Wu Z, Xie J. Lu X, et al. Evid Based Complement Alternat Med. 2016;2016:7954982. doi: 10.1155/2016/7954982. Epub 2016 Jun 14. Evid Based Complement Alternat Med. 2016. PMID: 27403199 Free PMC article. - Metabotropic glutamate receptor-8 relieves neonatal maternal separation-induced visceral hypersensitivity in rats by regulating expression of TNF-α.
Wang X, Shao L, Hua H, Chen Y. Wang X, et al. Ann Transl Med. 2023 Jan 31;11(2):118. doi: 10.21037/atm-22-6452. Ann Transl Med. 2023. PMID: 36819583 Free PMC article. - Early life stress disrupts intestinal homeostasis via NGF-TrkA signaling.
Wong HLX, Qin HY, Tsang SW, Zuo X, Che S, Chow CFW, Li X, Xiao HT, Zhao L, Huang T, Lin CY, Kwan HY, Yang T, Longo FM, Lyu A, Bian ZX. Wong HLX, et al. Nat Commun. 2019 Apr 15;10(1):1745. doi: 10.1038/s41467-019-09744-3. Nat Commun. 2019. PMID: 30988299 Free PMC article. - Role of neuronal nitric oxide synthase in colonic distension-induced hyperalgesia in distal colon of neonatal maternal separated male rats.
Tjong YW, Ip SP, Lao L, Wu J, Fong HH, Sung JJ, Berman B, Che CT. Tjong YW, et al. Neurogastroenterol Motil. 2011 Jul;23(7):666-e278. doi: 10.1111/j.1365-2982.2011.01697.x. Epub 2011 Mar 16. Neurogastroenterol Motil. 2011. PMID: 21410601 Free PMC article. - Effects of neonatal rearing by different types of foster mother on the distribution of corticotropin-releasing factor neurons in the central amygdaloid nucleus in rats.
Niina-Nakamura M, Kawano H. Niina-Nakamura M, et al. Exp Brain Res. 2022 Nov;240(11):2981-2988. doi: 10.1007/s00221-022-06468-7. Epub 2022 Oct 1. Exp Brain Res. 2022. PMID: 36181544
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical