Transient Notch signaling induces NK cell potential in Pax5-deficient pro-B cells - PubMed (original) (raw)
. 2006 Dec;36(12):3294-304.
doi: 10.1002/eji.200636325.
Affiliations
- PMID: 17111353
- DOI: 10.1002/eji.200636325
Free article
Transient Notch signaling induces NK cell potential in Pax5-deficient pro-B cells
Sebastian Carotta et al. Eur J Immunol. 2006 Dec.
Free article
Abstract
Unlike early B/T cell development, NK cell lineage commitment is not well understood, with a major limitation being the lack of a robust culture system to assay NK cell progenitors. Here we have exploited the multi-lineage potential of Pax5(-/-) pro-B cells to establish an effective system to direct differentiation of progenitors into the NK cell lineage. Cultivation of Pax5(-/-) pro-B cells on OP9 cells expressing the Notch ligand Delta-Like1 (OP9-DL1) in the presence of IL-7 efficiently induced T and NK cell potential. For NK cells, Notch was only transiently required, as prolonged signaling decreased NK and increased T cell development. Pure NK cell populations could be obtained by the culture of these Notch signal-experienced cells onto OP9 stroma and IL-15. A similar transient exposure to Notch was also compatible with the differentiation of NK cells from hematopoietic progenitors, while sustained Notch signaling impaired NK cell generation. Pax5(-/-) pro-B cell-derived NK cells were cytotoxic, secreted cytokines and expressed all the expected NK cell-specific surface markers examined except the Ly49 family, a phenotype similar to fetal NK cells. These data indicate that Notch signaling induces T/NK cell differentiation in Pax5(-/-) pro-B cells that is strikingly similar to early thymopoiesis.
Similar articles
- The potential involvement of Notch signaling in NK cell development.
Rolink AG, Balciunaite G, Demolière C, Ceredig R. Rolink AG, et al. Immunol Lett. 2006 Sep 15;107(1):50-7. doi: 10.1016/j.imlet.2006.07.005. Epub 2006 Aug 7. Immunol Lett. 2006. PMID: 16930724 - Critical role for c-kit (CD117) in T cell lineage commitment and early thymocyte development in vitro.
Massa S, Balciunaite G, Ceredig R, Rolink AG. Massa S, et al. Eur J Immunol. 2006 Mar;36(3):526-32. doi: 10.1002/eji.200535760. Eur J Immunol. 2006. PMID: 16482516 - Analysis of Notch1 function by in vitro T cell differentiation of Pax5 mutant lymphoid progenitors.
Höflinger S, Kesavan K, Fuxa M, Hutter C, Heavey B, Radtke F, Busslinger M. Höflinger S, et al. J Immunol. 2004 Sep 15;173(6):3935-44. doi: 10.4049/jimmunol.173.6.3935. J Immunol. 2004. PMID: 15356142 - Losing B cell identity.
Carotta S, Nutt SL. Carotta S, et al. Bioessays. 2008 Mar;30(3):203-7. doi: 10.1002/bies.20725. Bioessays. 2008. PMID: 18293359 Review. - Fidelity and infidelity in commitment to B-lymphocyte lineage development.
Rolink AG, Schaniel C, Busslinger M, Nutt SL, Melchers F. Rolink AG, et al. Immunol Rev. 2000 Jun;175:104-11. Immunol Rev. 2000. PMID: 10933595 Review.
Cited by
- Progression of Notch signaling regulation of B cells under radiation exposure.
Shu X, Wang J, Zeng H, Shao L. Shu X, et al. Front Immunol. 2024 Mar 8;15:1339977. doi: 10.3389/fimmu.2024.1339977. eCollection 2024. Front Immunol. 2024. PMID: 38524139 Free PMC article. Review. - Selective activation of STAT3 and STAT5 dictates the fate of myeloid progenitor cells.
Zhang M, Meng Y, Ying Y, Zhou P, Zhang S, Fang Y, Yao Y, Li D. Zhang M, et al. Cell Death Discov. 2023 Jul 28;9(1):274. doi: 10.1038/s41420-023-01575-y. Cell Death Discov. 2023. PMID: 37507383 Free PMC article. - Notch as an Immunologic Basis of Cancer Disparities.
LaLiah Thomas P, Shanker A. LaLiah Thomas P, et al. Cancer Health Disparities. 2019;3:e1-e10. Epub 2019 Aug 19. Cancer Health Disparities. 2019. PMID: 34268483 Free PMC article. - Transcriptional Regulation of Natural Killer Cell Development and Functions.
Wang D, Malarkannan S. Wang D, et al. Cancers (Basel). 2020 Jun 16;12(6):1591. doi: 10.3390/cancers12061591. Cancers (Basel). 2020. PMID: 32560225 Free PMC article. Review. - Determinant roles of dendritic cell-expressed Notch Delta-like and Jagged ligands on anti-tumor T cell immunity.
Tchekneva EE, Goruganthu MUL, Uzhachenko RV, Thomas PL, Antonucci A, Chekneva I, Koenig M, Piao L, Akhter A, de Aquino MTP, Ranganathan P, Long N, Magliery T, Valujskikh A, Evans JV, Arasada RR, Massion PP, Carbone DP, Shanker A, Dikov MM. Tchekneva EE, et al. J Immunother Cancer. 2019 Apr 2;7(1):95. doi: 10.1186/s40425-019-0566-4. J Immunother Cancer. 2019. PMID: 30940183 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Molecular Biology Databases