Molecular cloning and characterization of a ras p21-like GTP-binding protein (24KG) from rat liver - PubMed (original) (raw)

Comparative Study

. 1991 Jun 28;177(3):1224-32.

doi: 10.1016/0006-291x(91)90672-t.

Affiliations

• PMID: 1711847
• DOI: 10.1016/0006-291x(91)90672-t

Comparative Study

Molecular cloning and characterization of a ras p21-like GTP-binding protein (24KG) from rat liver

K Sakurada et al. Biochem Biophys Res Commun. 1991.

Abstract

We have isolated cDNA clones from a rat liver cDNA library that encode a ras p21-like small GTP-binding protein (24KG) which was purified from the microsomes-Golgi complex fraction of the rat liver. The cloning was accomplished using polymerase chain reaction amplified with a set of oligonucleotide primers which were designed from the partial amino acid sequences for 24KG. The cDNA contained an open reading frame encoding a 216 amino acid protein with a calculated Mr weight of 24,397. This Mr weight was similar to that of the purified 24KG estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The sequence analysis of 24KG revealed that a 24KG cDNA is the rat counterpart of a rab11 cDNA cloned from a Madin-Darby canine kidney cell cDNA library. The 1.0-kilobase 24KG mRNA corresponding to the isolated cDNA was also detected in various rat tissues, such as brain, testis, spleen, and heart.

PubMed Disclaimer

Similar articles

• Molecular cloning of the cDNA for stimulatory GDP/GTP exchange protein for smg p21s (ras p21-like small GTP-binding proteins) and characterization of stimulatory GDP/GTP exchange protein.
  Kaibuchi K, Mizuno T, Fujioka H, Yamamoto T, Kishi K, Fukumoto Y, Hori Y, Takai Y. Kaibuchi K, et al. Mol Cell Biol. 1991 May;11(5):2873-80. doi: 10.1128/mcb.11.5.2873-2880.1991. Mol Cell Biol. 1991. PMID: 1901951 Free PMC article.
• Molecular cloning and characterization of a novel type of regulatory protein (GDI) for smg p25A, a ras p21-like GTP-binding protein.
  Matsui Y, Kikuchi A, Araki S, Hata Y, Kondo J, Teranishi Y, Takai Y. Matsui Y, et al. Mol Cell Biol. 1990 Aug;10(8):4116-22. doi: 10.1128/mcb.10.8.4116-4122.1990. Mol Cell Biol. 1990. PMID: 2115118 Free PMC article.
• The ram: a novel low molecular weight GTP-binding protein cDNA from a rat megakaryocyte library.
  Nagata K, Satoh T, Itoh H, Kozasa T, Okano Y, Doi T, Kaziro Y, Nozawa Y. Nagata K, et al. FEBS Lett. 1990 Nov 26;275(1-2):29-32. doi: 10.1016/0014-5793(90)81431-m. FEBS Lett. 1990. PMID: 2124544
• Molecular cloning and characterization of a novel type of regulatory protein (GDI) for the rho proteins, ras p21-like small GTP-binding proteins.
  Fukumoto Y, Kaibuchi K, Hori Y, Fujioka H, Araki S, Ueda T, Kikuchi A, Takai Y. Fukumoto Y, et al. Oncogene. 1990 Sep;5(9):1321-8. Oncogene. 1990. PMID: 2120668
• Neuropeptide hormone receptors: strategies for identification.
  Richter D, Morley SD, Buck F, Meyerhof W. Richter D, et al. J Recept Res. 1991;11(1-4):483-505. doi: 10.3109/10799899109066423. J Recept Res. 1991. PMID: 1653349 Review.

Cited by

• Expression analysis of Rab11 during zebrafish embryonic development.
  Zhang H, Gao Y, Qian P, Dong Z, Hao W, Liu D, Duan X. Zhang H, et al. BMC Dev Biol. 2019 Dec 30;19(1):25. doi: 10.1186/s12861-019-0207-7. BMC Dev Biol. 2019. PMID: 31884948 Free PMC article.
• Crystal structure of the cGMP-dependent protein kinase II leucine zipper and Rab11b protein complex reveals molecular details of G-kinase-specific interactions.
  Reger AS, Yang MP, Koide-Yoshida S, Guo E, Mehta S, Yuasa K, Liu A, Casteel DE, Kim C. Reger AS, et al. J Biol Chem. 2014 Sep 12;289(37):25393-403. doi: 10.1074/jbc.M114.575894. Epub 2014 Jul 28. J Biol Chem. 2014. PMID: 25070890 Free PMC article.
• A novel tribasic Golgi export signal directs cargo protein interaction with activated Rab11 and AP-1-dependent Golgi-plasma membrane trafficking.
  Parmar HB, Duncan R. Parmar HB, et al. Mol Biol Cell. 2016 Apr 15;27(8):1320-31. doi: 10.1091/mbc.E15-12-0845. Epub 2016 Mar 3. Mol Biol Cell. 2016. PMID: 26941330 Free PMC article.
• Rab11a Mediates Vascular Endothelial-Cadherin Recycling and Controls Endothelial Barrier Function.
  Yan Z, Wang ZG, Segev N, Hu S, Minshall RD, Dull RO, Zhang M, Malik AB, Hu G. Yan Z, et al. Arterioscler Thromb Vasc Biol. 2016 Feb;36(2):339-49. doi: 10.1161/ATVBAHA.115.306549. Epub 2015 Dec 10. Arterioscler Thromb Vasc Biol. 2016. PMID: 26663395 Free PMC article.
• Rab11 is required for trans-golgi network-to-plasma membrane transport and a preferential target for GDP dissociation inhibitor.
  Chen W, Feng Y, Chen D, Wandinger-Ness A. Chen W, et al. Mol Biol Cell. 1998 Nov;9(11):3241-57. doi: 10.1091/mbc.9.11.3241. Mol Biol Cell. 1998. PMID: 9802909 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Molecular Biology Databases

  • Gene Ontology
  • GlyGen glycoinformatics resource