Signature-tagged mutagenesis: barcoding mutants for genome-wide screens - PubMed (original) (raw)

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doi: 10.1038/nrg1984.

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• PMID: 17139324
• DOI: 10.1038/nrg1984

Review

Signature-tagged mutagenesis: barcoding mutants for genome-wide screens

Piotr Mazurkiewicz et al. Nat Rev Genet. 2006 Dec.

Abstract

DNA signature tags (molecular barcodes) facilitate functional screens by identifying mutants in mixed populations that have a reduced or increased adaptation to a particular environment. Many innovative adaptations and refinements in the technology have been described since its original use with Salmonella; they have yielded a wealth of information on a broad range of biological processes--mainly in bacteria, but also in yeast and other fungi, viruses, parasites and, most recently, in mammalian cells. By combining whole-genome microarrays and comprehensive ordered libraries of mutants, high-throughput functional screens can now be achieved on a genomic scale.

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