Phosphorylation-regulated Cl- channel in CHO cells stably expressing the cystic fibrosis gene - PubMed (original) (raw)

. 1991 Aug 15;352(6336):628-31.

doi: 10.1038/352628a0.

Affiliations

• PMID: 1714039
• DOI: 10.1038/352628a0

Phosphorylation-regulated Cl- channel in CHO cells stably expressing the cystic fibrosis gene

J A Tabcharani et al. Nature. 1991.

Abstract

A cyclic AMP-stimulated chloride conductance appears when the cystic fibrosis gene is expressed in non-epithelial cells by infection with recombinant viruses. Cyclic AMP-stimulated conductance in this system is mediated by the same ohmic, low-conductance Cl- channel as in human secretory epithelia, but control of this channel by phosphorylation has not been directly demonstrated. Here we report the appearance of the low-conductance Cl- channel in Chinese hamster ovary cells after stable transfection with the cystic fibrosis gene. The channel is regulated on-cell by membrane-permeant analogues of cAMP and off-cell by protein kinases A and C and by alkaline phosphatase. These results are further evidence that the cystic fibrosis transmembrane regulator is a Cl- channel which can be activated by specific phosphorylation events and inactivated by dephosphorylation; they reveal an unsuspected synergism between converging kinase regulatory pathways.

PubMed Disclaimer

Similar articles

• Mutations in CFTR associated with mild-disease-form Cl- channels with altered pore properties.
  Sheppard DN, Rich DP, Ostedgaard LS, Gregory RJ, Smith AE, Welsh MJ. Sheppard DN, et al. Nature. 1993 Mar 11;362(6416):160-4. doi: 10.1038/362160a0. Nature. 1993. PMID: 7680769
• Defective regulation of outwardly rectifying Cl- channels by protein kinase A corrected by insertion of CFTR.
  Egan M, Flotte T, Afione S, Solow R, Zeitlin PL, Carter BJ, Guggino WB. Egan M, et al. Nature. 1992 Aug 13;358(6387):581-4. doi: 10.1038/358581a0. Nature. 1992. PMID: 1380129
• Phosphorylation fails to activate chloride channels from cystic fibrosis airway cells.
  Schoumacher RA, Shoemaker RL, Halm DR, Tallant EA, Wallace RW, Frizzell RA. Schoumacher RA, et al. Nature. 1987 Dec 24-31;330(6150):752-4. doi: 10.1038/330752a0. Nature. 1987. PMID: 2447502
• CFTR, chloride concentration and cell volume: could mammalian protein histidine phosphorylation play a latent role?
  Treharne KJ, Crawford RM, Mehta A. Treharne KJ, et al. Exp Physiol. 2006 Jan;91(1):131-9. doi: 10.1113/expphysiol.2005.031823. Epub 2005 Oct 11. Exp Physiol. 2006. PMID: 16219660 Review.
• Cystic fibrosis transmembrane conductance regulator (CFTR) and renal function.
  Stanton BA. Stanton BA. Wien Klin Wochenschr. 1997 Jun 27;109(12-13):457-64. Wien Klin Wochenschr. 1997. PMID: 9261986 Review.

Cited by

• Histamine 1 receptor-Gβγ-cAMP/PKA-CFTR pathway mediates the histamine-induced resetting of the suprachiasmatic circadian clock.
  Kim YS, Kim YB, Kim WB, Lee SW, Oh SB, Han HC, Lee CJ, Colwell CS, Kim YI. Kim YS, et al. Mol Brain. 2016 May 6;9(1):49. doi: 10.1186/s13041-016-0227-1. Mol Brain. 2016. PMID: 27153809 Free PMC article.
• Airway hydration and COPD.
  Ghosh A, Boucher RC, Tarran R. Ghosh A, et al. Cell Mol Life Sci. 2015 Oct;72(19):3637-52. doi: 10.1007/s00018-015-1946-7. Epub 2015 Jun 12. Cell Mol Life Sci. 2015. PMID: 26068443 Free PMC article. Review.
• The outwardly rectifying Cl- channel is not involved in cAMP-mediated Cl- secretion in HT-29 cells: evidence for a very-low-conductance Cl- channel.
  Fischer H, Kreusel KM, Illek B, Machen TE, Hegel U, Clauss W. Fischer H, et al. Pflugers Arch. 1992 Nov;422(2):159-67. doi: 10.1007/BF00370415. Pflugers Arch. 1992. PMID: 1283217
• cAMP-dependent activation of small-conductance Cl- channels in HT29 colon carcinoma cells.
  Kunzelmann K, Grolik M, Kubitz R, Greger R. Kunzelmann K, et al. Pflugers Arch. 1992 Jun;421(2-3):230-7. doi: 10.1007/BF00374832. Pflugers Arch. 1992. PMID: 1382267
• CFTR is required for PKA-regulated ATP sensitivity of Kir1.1 potassium channels in mouse kidney.
  Lu M, Leng Q, Egan ME, Caplan MJ, Boulpaep EL, Giebisch GH, Hebert SC. Lu M, et al. J Clin Invest. 2006 Mar;116(3):797-807. doi: 10.1172/JCI26961. Epub 2006 Feb 9. J Clin Invest. 2006. PMID: 16470247 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Nature Publishing Group

• Other Literature Sources

  • The Lens - Patent Citations Database

• Medical

  • Genetic Alliance
  • MedlinePlus Health Information