Cyclosporin A inhibits an initial step in folding of transferrin within the endoplasmic reticulum - PubMed (original) (raw)
. 1991 Aug 15;266(23):14835-8.
Affiliations
- PMID: 1714445
Free article
Cyclosporin A inhibits an initial step in folding of transferrin within the endoplasmic reticulum
H F Lodish et al. J Biol Chem. 1991.
Free article
Abstract
As resolved by electrophoresis in non-reducing SDS gels, transferrin newly made in Hep G2 cells migrates as a very diffuse set of species. During a subsequent 1-h chase all transferrin polypeptides are converted to a single, rapidly migrating species. These changes in gel mobility are due to alterations in the pattern of disulfide bonding, are not caused by carbohydrate processing, and occur while the protein is in the rough endoplasmic reticulum. Cyclosporin A causes an approximately 10-min lag in transferrin folding, after which folding resumes at the normal rate. Cyclosporin A also retards transferrin maturation from the endoplasmic reticulum and its secretion, at concentrations that do not affect secretion of other hepatoma proteins. Neither FK506 nor rapamycin affect transferrin folding. We conclude that an initial stage in transferrin folding is accelerated by an endoplasmic reticulum peptidyl-proline isomerase that is inhibited by cyclosporin A.
Similar articles
- Variability in transport rates of secretory glycoproteins through the endoplasmic reticulum and Golgi in human hepatoma cells.
Yeo KT, Parent JB, Yeo TK, Olden K. Yeo KT, et al. J Biol Chem. 1985 Jul 5;260(13):7896-902. J Biol Chem. 1985. PMID: 2989265 - Nuclear association of a T-cell transcription factor blocked by FK-506 and cyclosporin A.
Flanagan WM, Corthésy B, Bram RJ, Crabtree GR. Flanagan WM, et al. Nature. 1991 Aug 29;352(6338):803-7. doi: 10.1038/352803a0. Nature. 1991. PMID: 1715516 - Chemistry and biology of the immunophilins and their immunosuppressive ligands.
Schreiber SL. Schreiber SL. Science. 1991 Jan 18;251(4991):283-7. doi: 10.1126/science.1702904. Science. 1991. PMID: 1702904 Review. - When worlds collide: immunosuppressants meet protein phosphatases.
McKeon F. McKeon F. Cell. 1991 Sep 6;66(5):823-6. doi: 10.1016/0092-8674(91)90426-y. Cell. 1991. PMID: 1716181 Review. No abstract available.
Cited by
- Phosphorylation at serines 216 and 221 is important for Drosophila HeT-A Gag protein stability.
Brar SS, Petrovich RM, Williams JG, Mason JM. Brar SS, et al. PLoS One. 2013 Sep 18;8(9):e75381. doi: 10.1371/journal.pone.0075381. eCollection 2013. PLoS One. 2013. PMID: 24058682 Free PMC article. - Functions of FKBP12 and mitochondrial cyclophilin active site residues in vitro and in vivo in Saccharomyces cerevisiae.
Dolinski K, Scholz C, Muir RS, Rospert S, Schmid FX, Cardenas ME, Heitman J. Dolinski K, et al. Mol Biol Cell. 1997 Nov;8(11):2267-80. doi: 10.1091/mbc.8.11.2267. Mol Biol Cell. 1997. PMID: 9362068 Free PMC article. - The immunosuppressant FK506 inhibits amino acid import in Saccharomyces cerevisiae.
Heitman J, Koller A, Kunz J, Henriquez R, Schmidt A, Movva NR, Hall MN. Heitman J, et al. Mol Cell Biol. 1993 Aug;13(8):5010-9. doi: 10.1128/mcb.13.8.5010-5019.1993. Mol Cell Biol. 1993. PMID: 7687745 Free PMC article. - Yeast flavin-containing monooxygenase generates oxidizing equivalents that control protein folding in the endoplasmic reticulum.
Suh JK, Poulsen LL, Ziegler DM, Robertus JD. Suh JK, et al. Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):2687-91. doi: 10.1073/pnas.96.6.2687. Proc Natl Acad Sci U S A. 1999. PMID: 10077572 Free PMC article. - Evidence that cyclophilin-A protects cells against oxidative stress.
Doyle V, Virji S, Crompton M. Doyle V, et al. Biochem J. 1999 Jul 1;341 ( Pt 1)(Pt 1):127-32. Biochem J. 1999. PMID: 10377253 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials