Circadian oscillation of gene expression in murine calvarial bone - PubMed (original) (raw)
Comparative Study
doi: 10.1359/jbmr.061114.
Affiliations
- PMID: 17144790
- DOI: 10.1359/jbmr.061114
Free article
Comparative Study
Circadian oscillation of gene expression in murine calvarial bone
Sanjin Zvonic et al. J Bone Miner Res. 2007 Mar.
Free article
Abstract
The genes encoding the core circadian transcription factors display an oscillating expression profile in murine calvarial bone. More than 26% of the calvarial bone transcriptome exhibits a circadian rhythm, comparable with that observed in brown and white adipose tissues and liver. Thus, circadian mechanisms may directly modulate oxidative phosphorylation and multiple metabolic pathways in bone homeostasis.
Introduction: Although circadian rhythms have been associated historically with central regulatory mechanisms, there is emerging evidence that the circadian transcriptional apparatus exists in peripheral tissues. The aim of this study was to determine the presence and extent of circadian oscillation in the transcriptome of murine calvarial bone.
Materials and methods: Cohorts of 8-week-old male AKR/J mice were maintained in a controlled 12-h light:12-h dark cycle on an ad libitum diet for 2 weeks. Groups of three mice were killed every 4 h over a 48-h period. The level of gene expression at successive times-points was determined by quantitative RT-PCR and Affymetrix microarray. Data were analyzed using multiple statistical time series algorithms, including Cosinor, Fisher g-test, and the permutation time test.
Results: Both the positive (Bmal1, Npas2) and negative (Cry1, Cry2, Per1, Per2, Per3) elements of the circadian transcriptional apparatus and their immediate downstream targets and mediators (Dbp, Rev-erbalpha, Rev-erbbeta) exhibited oscillatory expression profiles. Consistent with findings in other tissues, the positive and negative elements were in antiphase relative to each other. More than 26% of the genes present on the microarray displayed an oscillatory profile in calvarial bone, comparable with the levels observed in brown and white adipose tissues and liver; however, only a subset of 174 oscillating genes were shared among all four tissues.
Conclusions: Our findings show that the components of the circadian transcriptional apparatus are represented in calvarial bone and display coordinated oscillatory behavior. However, these are not the only genes to display an oscillatory expression profile, which is seen in multiple pathways involving oxidative phosphorylation and lipid, protein, and carbohydrate metabolism.
Similar articles
- Differential patterns in the periodicity and dynamics of clock gene expression in mouse liver and stomach.
Mazzoccoli G, Francavilla M, Pazienza V, Benegiamo G, Piepoli A, Vinciguerra M, Giuliani F, Yamamoto T, Takumi T. Mazzoccoli G, et al. Chronobiol Int. 2012 Dec;29(10):1300-11. doi: 10.3109/07420528.2012.728662. Epub 2012 Nov 6. Chronobiol Int. 2012. PMID: 23131081 - Clock gene expression in mouse kidney and testis: analysis of periodical and dynamical patterns.
Mazzoccoli G, Francavilla M, Giuliani F, Aucella F, Vinciguerra M, Pazienza V, Piepoli A, Benegiamo G, Liu S, Cai Y. Mazzoccoli G, et al. J Biol Regul Homeost Agents. 2012 Apr-Jun;26(2):303-11. J Biol Regul Homeost Agents. 2012. PMID: 22824757 - Multiple mechanisms regulate circadian expression of the gene for cholesterol 7alpha-hydroxylase (Cyp7a), a key enzyme in hepatic bile acid biosynthesis.
Noshiro M, Usui E, Kawamoto T, Kubo H, Fujimoto K, Furukawa M, Honma S, Makishima M, Honma K, Kato Y. Noshiro M, et al. J Biol Rhythms. 2007 Aug;22(4):299-311. doi: 10.1177/0748730407302461. J Biol Rhythms. 2007. PMID: 17660447 - Melatonin feedback on clock genes: a theory involving the proteasome.
Vriend J, Reiter RJ. Vriend J, et al. J Pineal Res. 2015 Jan;58(1):1-11. doi: 10.1111/jpi.12189. Epub 2014 Nov 22. J Pineal Res. 2015. PMID: 25369242 Review. - Dissecting the Rev-erbα Cistrome and the Mechanisms Controlling Circadian Transcription in Liver.
Fang B, Lazar MA. Fang B, et al. Cold Spring Harb Symp Quant Biol. 2015;80:233-8. doi: 10.1101/sqb.2015.80.027508. Epub 2015 Sep 14. Cold Spring Harb Symp Quant Biol. 2015. PMID: 26370410 Review.
Cited by
- Biological aging alters circadian mechanisms in murine adipose tissue depots.
Sutton GM, Ptitsyn AA, Floyd ZE, Yu G, Wu X, Hamel K, Shah FS, Centanni A, Eilertsen K, Kheterpal I, Newman S, Leonardi C, Freitas MA, Bunnell BA, Gimble JM. Sutton GM, et al. Age (Dordr). 2013 Jun;35(3):533-47. doi: 10.1007/s11357-012-9389-7. Epub 2012 Mar 13. Age (Dordr). 2013. PMID: 22411258 Free PMC article. - Tracking circadian rhythms of bone mineral deposition in murine calvarial organ cultures.
McElderry JD, Zhao G, Khmaladze A, Wilson CG, Franceschi RT, Morris MD. McElderry JD, et al. J Bone Miner Res. 2013 Aug;28(8):1846-54. doi: 10.1002/jbmr.1924. J Bone Miner Res. 2013. PMID: 23505073 Free PMC article. - BMAL1 deficiency promotes skeletal mandibular hypoplasia via OPG downregulation.
Zhou X, Yu R, Long Y, Zhao J, Yu S, Tang Q, Chen L. Zhou X, et al. Cell Prolif. 2018 Oct;51(5):e12470. doi: 10.1111/cpr.12470. Epub 2018 Aug 17. Cell Prolif. 2018. PMID: 30117209 Free PMC article. - Chronobiology and Chronotherapy of Osteoporosis.
Winter EM, Kooijman S, Appelman-Dijkstra NM, Meijer OC, Rensen PC, Schilperoort M. Winter EM, et al. JBMR Plus. 2021 May 5;5(10):e10504. doi: 10.1002/jbm4.10504. eCollection 2021 Oct. JBMR Plus. 2021. PMID: 34693186 Free PMC article. Review. - Deletion of clock gene Bmal1 impaired the chondrocyte function due to disruption of the HIF1α-VEGF signaling pathway.
Ma Z, Jin X, Qian Z, Li F, Xu M, Zhang Y, Kang X, Li H, Gao X, Zhao L, Zhang Z, Zhang Y, Wu S, Sun H. Ma Z, et al. Cell Cycle. 2019 Jul;18(13):1473-1489. doi: 10.1080/15384101.2019.1620572. Epub 2019 May 26. Cell Cycle. 2019. PMID: 31107137 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources