Genome-wide distribution of yeast RNA polymerase II and its control by Sen1 helicase - PubMed (original) (raw)
Comparative Study
. 2006 Dec 8;24(5):735-746.
doi: 10.1016/j.molcel.2006.10.023.
Affiliations
- PMID: 17157256
- DOI: 10.1016/j.molcel.2006.10.023
Free article
Comparative Study
Genome-wide distribution of yeast RNA polymerase II and its control by Sen1 helicase
Eric J Steinmetz et al. Mol Cell. 2006.
Free article
Abstract
Functional engagement of RNA polymerase II (Pol II) with eukaryotic chromosomes is a fundamental and highly regulated biological process. Here we present a high-resolution map of Pol II occupancy across the entire yeast genome. We compared a wild-type strain with a strain bearing a substitution in the Sen1 helicase, which is a Pol II termination factor for noncoding RNA genes. The wild-type pattern of Pol II distribution provides unexpected insights into the mechanisms by which genes are repressed or silenced. Remarkably, a single amino acid substitution that compromises Sen1 function causes profound changes in Pol II distribution over both noncoding and protein-coding genes, establishing an important function of Sen1 in the regulation of transcription. Given the strong similarity of the yeast and human Sen1 proteins, our results suggest that progressive neurological disorders caused by substitutions in the human Sen1 homolog Senataxin may be due to misregulation of transcription.
Similar articles
- Transcriptomes of six mutants in the Sen1 pathway reveal combinatorial control of transcription termination across the Saccharomyces cerevisiae genome.
Chen X, Poorey K, Carver MN, Müller U, Bekiranov S, Auble DT, Brow DA. Chen X, et al. PLoS Genet. 2017 Jun 30;13(6):e1006863. doi: 10.1371/journal.pgen.1006863. eCollection 2017 Jun. PLoS Genet. 2017. PMID: 28665995 Free PMC article. - Kinetic competition between RNA Polymerase II and Sen1-dependent transcription termination.
Hazelbaker DZ, Marquardt S, Wlotzka W, Buratowski S. Hazelbaker DZ, et al. Mol Cell. 2013 Jan 10;49(1):55-66. doi: 10.1016/j.molcel.2012.10.014. Epub 2012 Nov 21. Mol Cell. 2013. PMID: 23177741 Free PMC article. - RNA processing factors Swd2.2 and Sen1 antagonize RNA Pol III-dependent transcription and the localization of condensin at Pol III genes.
Legros P, Malapert A, Niinuma S, Bernard P, Vanoosthuyse V. Legros P, et al. PLoS Genet. 2014 Nov 13;10(11):e1004794. doi: 10.1371/journal.pgen.1004794. eCollection 2014 Nov. PLoS Genet. 2014. PMID: 25392932 Free PMC article. - The Nrd1-Nab3-Sen1 transcription termination complex from a structural perspective.
Chaves-Arquero B, Pérez-Cañadillas JM. Chaves-Arquero B, et al. Biochem Soc Trans. 2023 Jun 28;51(3):1257-1269. doi: 10.1042/BST20221418. Biochem Soc Trans. 2023. PMID: 37222282 Free PMC article. Review. - Genome-wide RNA polymerase II: not genes only!
Koch F, Jourquin F, Ferrier P, Andrau JC. Koch F, et al. Trends Biochem Sci. 2008 Jun;33(6):265-73. doi: 10.1016/j.tibs.2008.04.006. Epub 2008 May 6. Trends Biochem Sci. 2008. PMID: 18467100 Review.
Cited by
- Unraveling the mechanistic features of RNA polymerase II termination by the 5'-3' exoribonuclease Rat1.
Park J, Kang M, Kim M. Park J, et al. Nucleic Acids Res. 2015 Mar 11;43(5):2625-37. doi: 10.1093/nar/gkv133. Epub 2015 Feb 26. Nucleic Acids Res. 2015. PMID: 25722373 Free PMC article. - Interactions of Sen1, Nrd1, and Nab3 with multiple phosphorylated forms of the Rpb1 C-terminal domain in Saccharomyces cerevisiae.
Chinchilla K, Rodriguez-Molina JB, Ursic D, Finkel JS, Ansari AZ, Culbertson MR. Chinchilla K, et al. Eukaryot Cell. 2012 Apr;11(4):417-29. doi: 10.1128/EC.05320-11. Epub 2012 Jan 27. Eukaryot Cell. 2012. PMID: 22286094 Free PMC article. - The exosome component Rrp6 is required for RNA polymerase II termination at specific targets of the Nrd1-Nab3 pathway.
Fox MJ, Gao H, Smith-Kinnaman WR, Liu Y, Mosley AL. Fox MJ, et al. PLoS Genet. 2015 Feb 13;11(2):e1004999. doi: 10.1371/journal.pgen.1004999. eCollection 2015. PLoS Genet. 2015. PMID: 25680078 Free PMC article. - Gene loops enhance transcriptional directionality.
Tan-Wong SM, Zaugg JB, Camblong J, Xu Z, Zhang DW, Mischo HE, Ansari AZ, Luscombe NM, Steinmetz LM, Proudfoot NJ. Tan-Wong SM, et al. Science. 2012 Nov 2;338(6107):671-5. doi: 10.1126/science.1224350. Epub 2012 Sep 27. Science. 2012. PMID: 23019609 Free PMC article. - Endogenous single-strand DNA breaks at RNA polymerase II promoters in Saccharomyces cerevisiae.
Hegedüs É, Kókai E, Nánási P, Imre L, Halász L, Jossé R, Antunovics Z, Webb MR, El Hage A, Pommier Y, Székvölgyi L, Dombrádi V, Szabó G. Hegedüs É, et al. Nucleic Acids Res. 2018 Nov 16;46(20):10649-10668. doi: 10.1093/nar/gky743. Nucleic Acids Res. 2018. PMID: 30445637 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases