Protein array autoantibody profiles for insights into systemic lupus erythematosus and incomplete lupus syndromes - PubMed (original) (raw)

Average normalized fluorescent intensity (nfi) of the autoantibodies for the four subject groups are plotted. Reactivities of the seven IgG autoantibody clusters, as defined in Fig. 1a. Cluster 1 contains eight antibodies (β2-microglobulin, Ro/SSA 50KDa, Ro/SSA 62KDa, La/SSB, Jo-1, threonyl-tRNA synthetase and alanyl-tRNA synthetase); cluster 2 contains 12 antibodies (hyaluronic acid, Goodpasture antigen, heparin, haemocyanin, gliadin, fibrinogen S, fibrinogen IV, thyroid peroxidase, thyroglobulin, matrigel, laminin and heparin sulphate proteoglycan); cluster 3 contains 12 antibodies (collagen I, collagen II, collagen III, cytochrome C, myosin, PM/Scl100, CENP-B, CENP-A, proliferating cell nuclear antigen, vitronectin, tissue transglutaminase and intrinsic factor); cluster 4 contains seven DNA-related antibodies (chromatin, dsDNA, ssDNA, glomerular sonicate, Ku, GBM disassociated, collagen IV); cluster 5 contains eight antibodies (U1-snRNP-A, U1-snRNP-BB′, U1-snRNP-C, U1-snRNP-68, LC1, GBM, β2-glycoprotein I and ribosomal phosphoprotein P0); cluster 6 contains nine antibodies (total histone, histones H1, H2A, H2B, H3, H4, C1q, Scl70 and vimentin); cluster 8 contains two antibodies (cardolipin and chondroitin sulphate). A one-way analysis of variance (

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) non-parametric test was performed to compare all groups with non-autoimmune controls (NC) or with systemic lupus erythematosus (SLE), and the results are listed under each plot. P1 represents the comparison of each group to the NC group; P2 compares each group to SLE. *P < 0·05; **P < 0·01; ***P < 0·001, n.s.: not significant. Correlations between the seven IgG autoantibodies are shown in the final panel; values represent Pearson's r.