Rapid, small-scale RNA isolation from tissue culture cells - PubMed (original) (raw)
. 1991 Sep;11(3):324, 326-7.
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- PMID: 1718328
Rapid, small-scale RNA isolation from tissue culture cells
W Q Xie et al. Biotechniques. 1991 Sep.
Abstract
A rapid and simple protocol for the isolation of RNA from transfected tissue culture cells is described. The protocol employs a guanidinium thiocyanate/phenol mixture to lyse cells directly from tissue culture plates and extract the total RNA. A total of six simple steps, which can be accomplished within 2.5 hours, are required. The protocol reproducibly yields 20-40 micrograms RNA from 0.5 x 10(6) - 1 x 10(6) cells per sample. The quality of the RNA obtained is sufficient for reverse transcriptase assays such as oligonucleotide-directed primer extension and random-primed cDNA synthesis.
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