Human airway surface epithelial regeneration is delayed and abnormal in cystic fibrosis - PubMed (original) (raw)
. 2007 Feb;211(3):340-50.
doi: 10.1002/path.2118.
Affiliations
- PMID: 17186573
- DOI: 10.1002/path.2118
Human airway surface epithelial regeneration is delayed and abnormal in cystic fibrosis
R Hajj et al. J Pathol. 2007 Feb.
Abstract
Cystic fibrosis (CF) at an advanced stage of the disease is characterized by airway epithelial injury and remodelling. Whether CF remodelling is related to infection and inflammation or due to an abnormal regenerative process is still undecided. We have recently established the expression and secretion profiles of interleukin (IL)-8, matrix metalloproteinase (MMP)-7, MMP-9, and tissue inhibitor of metalloproteinase (TIMP)-1 during non-CF airway epithelial regeneration in a humanized nude mouse xenograft model. To enhance our understanding of CF remodelling, we compared the regeneration process of non-infected human CF and non-CF nasal epithelia. In both CF and non-CF situations, epithelial regeneration was characterized by successive steps of cell adhesion and migration, proliferation, pseudostratification, and terminal differentiation. However, histological examination of the grafts showed a delay in differentiation of the CF airway epithelium. Cell proliferation was higher in the regenerating CF epithelium, and the differentiated CF epithelium exhibited a pronounced height increase and basal cell hyperplasia in comparison with non-CF epithelium. In addition, while the number of goblet cells expressing MUC5AC was similar in CF and non-CF regenerated epithelia, the number of MUC5B-immunopositive goblet cells was lower in CF grafts. The expression of human IL-8, MMP-7, MMP-9, and TIMP-1 was enhanced in CF epithelium, especially early in the regenerative process. Together, our data strongly suggest that the regeneration of human CF airway surface epithelium is characterized by remodelling, delayed differentiation, and altered pro-inflammatory and MMP responses.
Copyright 2006 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Similar articles
- Differential expression of matrix metalloproteinases and interleukin-8 during regeneration of human airway epithelium in vivo.
Coraux C, Martinella-Catusse C, Nawrocki-Raby B, Hajj R, Burlet H, Escotte S, Laplace V, Birembaut P, Puchelle E. Coraux C, et al. J Pathol. 2005 Jun;206(2):160-9. doi: 10.1002/path.1757. J Pathol. 2005. PMID: 15806599 - Induced sputum matrix metalloproteinase-9 correlates with lung function and airway inflammation in children with cystic fibrosis.
Sagel SD, Kapsner RK, Osberg I. Sagel SD, et al. Pediatr Pulmonol. 2005 Mar;39(3):224-32. doi: 10.1002/ppul.20165. Pediatr Pulmonol. 2005. PMID: 15635615 Clinical Trial. - Cystic fibrosis airway epithelium remodelling: involvement of inflammation.
Adam D, Roux-Delrieu J, Luczka E, Bonnomet A, Lesage J, Mérol JC, Polette M, Abély M, Coraux C. Adam D, et al. J Pathol. 2015 Feb;235(3):408-19. doi: 10.1002/path.4471. Epub 2014 Dec 18. J Pathol. 2015. PMID: 25348090 - Reconstitution of human airway tissue in the humanized xenograft model.
Escotte S, Catusse C, Coraux C, Puchelle E. Escotte S, et al. J Cyst Fibros. 2004 Aug;3 Suppl 2:63-5. doi: 10.1016/j.jcf.2004.05.014. J Cyst Fibros. 2004. PMID: 15463929 Review. - Human airway xenograft models of epithelial cell regeneration.
Puchelle E, Peault B. Puchelle E, et al. Respir Res. 2000;1(3):125-8. doi: 10.1186/rr21. Epub 2000 Oct 12. Respir Res. 2000. PMID: 11667974 Free PMC article. Review.
Cited by
- Lung immunoglobulin A immunity dysregulation in cystic fibrosis.
Collin AM, Lecocq M, Noel S, Detry B, Carlier FM, Aboubakar Nana F, Bouzin C, Leal T, Vermeersch M, De Rose V, Regard L, Martin C, Burgel PR, Hoton D, Verleden S, Froidure A, Pilette C, Gohy S. Collin AM, et al. EBioMedicine. 2020 Oct;60:102974. doi: 10.1016/j.ebiom.2020.102974. Epub 2020 Sep 11. EBioMedicine. 2020. PMID: 32927272 Free PMC article. - Bone marrow-derived progenitor cells in end-stage lung disease patients.
Gilpin SE, Lung K, de Couto GT, Cypel M, Sato M, Singer LG, Keshavjee S, Waddell TK. Gilpin SE, et al. BMC Pulm Med. 2013 Aug 3;13:48. doi: 10.1186/1471-2466-13-48. BMC Pulm Med. 2013. PMID: 23915095 Free PMC article. - Mutant CFTR Drives TWIST1 mediated epithelial-mesenchymal transition.
Quaresma MC, Pankonien I, Clarke LA, Sousa LS, Silva IAL, Railean V, Doušová T, Fuxe J, Amaral MD. Quaresma MC, et al. Cell Death Dis. 2020 Oct 26;11(10):920. doi: 10.1038/s41419-020-03119-z. Cell Death Dis. 2020. PMID: 33106471 Free PMC article. - Targeting the EGFR-ERK axis using the compatible solute ectoine to stabilize CFTR mutant F508del.
Wellmerling J, Rayner RE, Chang SW, Kairis EL, Kim SH, Sharma A, Boyaka PN, Cormet-Boyaka E. Wellmerling J, et al. FASEB J. 2022 May;36(5):e22270. doi: 10.1096/fj.202100458RRR. FASEB J. 2022. PMID: 35412656 Free PMC article. - The role of Lipoxin A4 in Cystic Fibrosis Lung Disease.
Urbach V, Higgins G, Buchanan P, Ringholz F. Urbach V, et al. Comput Struct Biotechnol J. 2013 Dec 6;6:e201303018. doi: 10.5936/csbj.201303018. eCollection 2013. Comput Struct Biotechnol J. 2013. PMID: 24688726 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials
Miscellaneous