t(8;21) breakpoints on chromosome 21 in acute myeloid leukemia are clustered within a limited region of a single gene, AML1 - PubMed (original) (raw)

t(8;21) breakpoints on chromosome 21 in acute myeloid leukemia are clustered within a limited region of a single gene, AML1

H Miyoshi et al. Proc Natl Acad Sci U S A. 1991.

Abstract

The t(8;21)(q22;q22) translocation is a non-random chromosomal abnormality frequently found in patients with acute myeloid leukemia (AML) with maturation (M2 subtype). We report here the cloning of a gene, named AML1, on chromosome 21 that was found to be rearranged in the leukemic cell DNAs from t(8;21) AML patients. The breakpoints in 16 out of 21 patients were clustered within a limited region of AML1, and detailed analysis in 3 patients revealed that the breakpoints occurred in the same intron of the gene. Sequencing of cDNA clones identified a long open reading frame encoding a 250-amino acid protein. Northern blot analysis detected four constant mRNA species in t(8;21) leukemic and normal cells; the largest species was more abundant in the leukemic cells than in normal cells. In addition, two mRNA species limited to the leukemic cells were found. These findings indicate that the AML1 gene may be involved in neoplastic transformation of AML with the t(8;21) translocation.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Nature. 1990 Oct 11;347(6293):558-61 - PubMed
    1. Anal Biochem. 1987 Apr;162(1):156-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1985 Jan;82(2):464-8 - PubMed
    1. N Engl J Med. 1988 Oct 13;319(15):990-8 - PubMed
    1. Trends Genet. 1988 Nov;4(11):300-4 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources