The xbp-1 gene is essential for development in Drosophila - PubMed (original) (raw)
. 2007 Feb;217(2):159-67.
doi: 10.1007/s00427-006-0124-1. Epub 2007 Jan 6.
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- PMID: 17206451
- DOI: 10.1007/s00427-006-0124-1
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The xbp-1 gene is essential for development in Drosophila
Sami Souid et al. Dev Genes Evol. 2007 Feb.
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Abstract
We report in this paper the characterization of Dxbp-1, the Drosophila homologue of the xpb-1 gene that encodes a "bZIP"-containing transcription factor that plays a key role in the unfolded protein response (UPR), an evolutionarily conserved signalling pathway activated by an overload of misfolded proteins in the endoplasmic reticulum (ER). Dxbp-1 is ubiquitously transcribed, and high levels are found in embryonic salivary glands and in the ovarian follicle cells committed to the synthesis of the respiratory appendages. Loss of function of Dxbp-1 induced a recessive larval lethality, thus, revealing an essential requirement for this gene. The Dxbp-1 transcript was submitted to an "unconventional" splicing that generated a processed Dxbp-1s transcript encoding a DXbp-1 protein isoform, as is the case for yeast, Caenorhabditis elegans and vertebrate hac1/xbp-1 transcripts after UPR activation. However, in the absence of exogenously induced ER stress, the Dxbp-1s transcript was also detectable not only throughout embryonic and larval development but also in adults with a high level of accumulation in the male sexual apparatus and, to a lesser extent, in the salivary glands of the third-instar larvae. Using a Dxbp-1:GFP transgene as an in vivo reporter for Dxbp-1 mRNA unconventional splicing, we confirmed that Dxbp-1 processing took place in the salivary glands of the third-instar larvae. The Dxbp-1 gene appears, thus, to play an essential role during the development of Drosophila, hypothetically by stimulating the folding capacities of the ER in cells committed to intense secretory activities.
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References
- Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4660-5 - PubMed
- Nat Immunol. 2003 Apr;4(4):321-9 - PubMed
- Science. 2000 Jan 28;287(5453):664-6 - PubMed
- Mol Cell Biol. 2003 Nov;23(21):7448-59 - PubMed
- Mech Dev. 2006 Jan;123(1):84-96 - PubMed
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