Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor - PubMed (original) (raw)
doi: 10.1038/nmeth1009. Epub 2007 Jan 28.
Affiliations
- PMID: 17259991
- DOI: 10.1038/nmeth1009
Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor
Nicola Heim et al. Nat Methods. 2007 Feb.
Abstract
Fluorescent Ca(2+) indicator proteins (FCIPs) are attractive tools for studying Ca(2+) dynamics in live cells. Here we describe transgenic mouse lines expressing a troponin C (TnC)-based biosensor. The biosensor is widely expressed in neurons and has improved Ca(2+) sensitivity both in vitro and in vivo. This allows FCIP-based two-photon Ca(2+) imaging of distinct neurons and their dendrites in vivo, and opens a new avenue for structure-function analysis of intact neuronal circuits.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous