Electron cryomicroscopy comparison of the architectures of the enveloped bacteriophages phi6 and phi8 - PubMed (original) (raw)
Comparative Study
Electron cryomicroscopy comparison of the architectures of the enveloped bacteriophages phi6 and phi8
Harri T Jäälinoja et al. Structure. 2007 Feb.
Free article
Abstract
The enveloped dsRNA bacteriophages phi6 and phi8 are the two most distantly related members of the Cystoviridae family. Their structure and function are similar to that of the Reoviridae but their assembly can be conveniently studied in vitro. Electron cryomicroscopy and three-dimensional icosahedral reconstruction were used to determine the structures of the phi6 virion (14 A resolution), phi8 virion (18 A resolution), and phi8 core (8.5 A resolution). Spikes protrude 2 nm from the membrane bilayer in phi6 and 7 nm in phi8. In the phi6 nucleocapsid, 600 copies of P8 and 72 copies of P4 interact with the membrane, whereas in phi8 it is only P4 and 60 copies of a minor protein. The major polymerase complex protein P1 forms a dodecahedral shell from 60 asymmetric dimers in both viruses, but the alpha-helical fold has apparently diverged. These structural differences reflect the different host ranges and entry and assembly mechanisms of the two viruses.
Similar articles
- A symmetry mismatch at the site of RNA packaging in the polymerase complex of dsRNA bacteriophage phi6.
de Haas F, Paatero AO, Mindich L, Bamford DH, Fuller SD. de Haas F, et al. J Mol Biol. 1999 Nov 26;294(2):357-72. doi: 10.1006/jmbi.1999.3260. J Mol Biol. 1999. PMID: 10610764 - Self-assembly of double-stranded RNA bacteriophages.
Poranen MM, Tuma R. Poranen MM, et al. Virus Res. 2004 Apr;101(1):93-100. doi: 10.1016/j.virusres.2003.12.009. Virus Res. 2004. PMID: 15010220 Review. - Characterization of phi12, a bacteriophage related to phi6: nucleotide sequence of the large double-stranded RNA.
Gottlieb P, Potgieter C, Wei H, Toporovsky I. Gottlieb P, et al. Virology. 2002 Apr 10;295(2):266-71. doi: 10.1006/viro.2002.1436. Virology. 2002. PMID: 12033785 - Structure of the bacteriophage phi6 nucleocapsid suggests a mechanism for sequential RNA packaging.
Huiskonen JT, de Haas F, Bubeck D, Bamford DH, Fuller SD, Butcher SJ. Huiskonen JT, et al. Structure. 2006 Jun;14(6):1039-48. doi: 10.1016/j.str.2006.03.018. Structure. 2006. PMID: 16765897 - RNA-dependent RNA polymerases of dsRNA bacteriophages.
Makeyev EV, Grimes JM. Makeyev EV, et al. Virus Res. 2004 Apr;101(1):45-55. doi: 10.1016/j.virusres.2003.12.005. Virus Res. 2004. PMID: 15010216 Review.
Cited by
- Combined approaches to flexible fitting and assessment in virus capsids undergoing conformational change.
Pandurangan AP, Shakeel S, Butcher SJ, Topf M. Pandurangan AP, et al. J Struct Biol. 2014 Mar;185(3):427-39. doi: 10.1016/j.jsb.2013.12.003. Epub 2013 Dec 12. J Struct Biol. 2014. PMID: 24333899 Free PMC article. - Virucidal or Not Virucidal? That Is the Question-Predictability of Ionic Liquid's Virucidal Potential in Biological Test Systems.
Sommer J, Fister S, Gundolf T, Bromberger B, Mester PJ, Witte AK, Kalb R, Rossmanith P. Sommer J, et al. Int J Mol Sci. 2018 Mar 9;19(3):790. doi: 10.3390/ijms19030790. Int J Mol Sci. 2018. PMID: 29522483 Free PMC article. - The T=1 capsid protein of Penicillium chrysogenum virus is formed by a repeated helix-rich core indicative of gene duplication.
Luque D, González JM, Garriga D, Ghabrial SA, Havens WM, Trus B, Verdaguer N, Carrascosa JL, Castón JR. Luque D, et al. J Virol. 2010 Jul;84(14):7256-66. doi: 10.1128/JVI.00432-10. Epub 2010 May 12. J Virol. 2010. PMID: 20463071 Free PMC article. - Atomic structure reveals the unique capsid organization of a dsRNA virus.
Pan J, Dong L, Lin L, Ochoa WF, Sinkovits RS, Havens WM, Nibert ML, Baker TS, Ghabrial SA, Tao YJ. Pan J, et al. Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4225-30. doi: 10.1073/pnas.0812071106. Epub 2009 Feb 25. Proc Natl Acad Sci U S A. 2009. PMID: 19246376 Free PMC article. - Three-dimensional structure of the enveloped bacteriophage phi12: an incomplete T = 13 lattice is superposed on an enclosed T = 1 shell.
Wei H, Cheng RH, Berriman J, Rice WJ, Stokes DL, Katz A, Morgan DG, Gottlieb P. Wei H, et al. PLoS One. 2009 Sep 3;4(9):e6850. doi: 10.1371/journal.pone.0006850. PLoS One. 2009. PMID: 19727406 Free PMC article.