Nanofabricated quartz cylinders for angular trapping: DNA supercoiling torque detection - PubMed (original) (raw)
doi: 10.1038/nmeth1013. Epub 2007 Feb 25.
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- PMID: 17322891
- DOI: 10.1038/nmeth1013
Nanofabricated quartz cylinders for angular trapping: DNA supercoiling torque detection
Christopher Deufel et al. Nat Methods. 2007 Mar.
Abstract
We designed and created nanofabricated quartz cylinders well suited for torque application and detection in an angular optical trap. We made the cylinder axis perpendicular to the extraordinary axis of the quartz crystal and chemically functionalized only one end of each cylinder for attachment to a DNA molecule. We directly measured the torque on a single DNA molecule as it underwent a phase transition from B-form to supercoiled P-form.
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