Actin-binding proteins coronin-1a and IBA-1 are effective microglial markers for immunohistochemistry - PubMed (original) (raw)

Comparative Study

. 2007 Jul;55(7):687-700.

doi: 10.1369/jhc.6A7156.2007. Epub 2007 Mar 6.

Affiliations

Comparative Study

Actin-binding proteins coronin-1a and IBA-1 are effective microglial markers for immunohistochemistry

Zeshan Ahmed et al. J Histochem Cytochem. 2007 Jul.

Abstract

This study identifies the actin-binding protein, coronin-1a, as a novel and effective immunohistochemical marker for microglia in both cell cultures and in formaldehyde-fixed, paraffin-embedded tissue. Antibodies to coronin-1a effectively immunostained microglia in human, monkey, horse, rat, and mouse tissues, even in tissues stored for long periods of time. The identity of coronin-1a-immunoreactive cells as microglia was confirmed using double immunolabeling with cell type-specific markers as well as by morphological features and the distribution of immunoreactive cells. These properties are shared by another actin-binding protein, IBA-1. Unlike IBA-1, coronin-1a immunoreactivity was also detected in lymphocytes and certain other hematopoietic cells. The results indicate that both coronin-1a and IBA-1 are robust markers for microglia that can be used in routinely processed tissue of humans and animals. Because both coronin-1a and IBA-1 are actin-binding proteins that play a role in rearrangement of the membrane cytoskeleton, it suggests that these proteins are critical to dynamic properties of microglia.

PubMed Disclaimer

Similar articles

Cited by

Publication types

MeSH terms

Substances

Grants and funding

LinkOut - more resources