Type III restriction enzymes need two inversely oriented recognition sites for DNA cleavage - PubMed (original) (raw)

. 1992 Jan 30;355(6359):467-9.

doi: 10.1038/355467a0.

Affiliations

• PMID: 1734285
• DOI: 10.1038/355467a0

Type III restriction enzymes need two inversely oriented recognition sites for DNA cleavage

A Meisel et al. Nature. 1992.

Abstract

Type III restriction/modification enzyme recognize short, non-palindromic sequences that can be methylated on only one strand, with the paradoxical consequence that during replication of what is in effect hemimethylated DNA totally unmodified sites arise. Why the unmodified sites are not subject to suicidal restriction was not clear. Here we show that restriction requires two unmodified recognition sites that can be separated by different distances but which must be in inverse orientation. All of the unmodified sites in newly replicated DNA are of course in the same orientation, which explains why they are not restricted. This result may be of relevance to other manifestations of anisotropy in double-stranded DNA, such as genetic imprinting.

PubMed Disclaimer

Similar articles

• DNA cleavage by type III restriction-modification enzyme EcoP15I is independent of spacer distance between two head to head oriented recognition sites.
  Mücke M, Reich S, Möncke-Buchner E, Reuter M, Krüger DH. Mücke M, et al. J Mol Biol. 2001 Sep 28;312(4):687-98. doi: 10.1006/jmbi.2001.4998. J Mol Biol. 2001. PMID: 11575924
• DNA cleavage by the type IC restriction-modification enzyme EcoR124II.
  Dreier J, MacWilliams MP, Bickle TA. Dreier J, et al. J Mol Biol. 1996 Dec 13;264(4):722-33. doi: 10.1006/jmbi.1996.0672. J Mol Biol. 1996. PMID: 8980681
• Subunit assembly and mode of DNA cleavage of the type III restriction endonucleases EcoP1I and EcoP15I.
  Janscak P, Sandmeier U, Szczelkun MD, Bickle TA. Janscak P, et al. J Mol Biol. 2001 Feb 23;306(3):417-31. doi: 10.1006/jmbi.2000.4411. J Mol Biol. 2001. PMID: 11178902
• Complex restriction enzymes: NTP-driven molecular motors.
  Bourniquel AA, Bickle TA. Bourniquel AA, et al. Biochimie. 2002 Nov;84(11):1047-59. doi: 10.1016/s0300-9084(02)00020-2. Biochimie. 2002. PMID: 12595133 Review.
• The significance of distance and orientation of restriction endonuclease recognition sites in viral DNA genomes.
  Krüger DH, Kupper D, Meisel A, Reuter M, Schroeder C. Krüger DH, et al. FEMS Microbiol Rev. 1995 Aug;17(1-2):177-84. doi: 10.1111/j.1574-6976.1995.tb00200.x. FEMS Microbiol Rev. 1995. PMID: 7669344 Review.

Cited by

• Digital gene expression for non-model organisms.
  Hong LZ, Li J, Schmidt-Küntzel A, Warren WC, Barsh GS. Hong LZ, et al. Genome Res. 2011 Nov;21(11):1905-15. doi: 10.1101/gr.122135.111. Epub 2011 Aug 15. Genome Res. 2011. PMID: 21844123 Free PMC article.
• Functional analysis of MmeI from methanol utilizer Methylophilus methylotrophus, a subtype IIC restriction-modification enzyme related to type I enzymes.
  Nakonieczna J, Kaczorowski T, Obarska-Kosinska A, Bujnicki JM. Nakonieczna J, et al. Appl Environ Microbiol. 2009 Jan;75(1):212-23. doi: 10.1128/AEM.01322-08. Epub 2008 Nov 7. Appl Environ Microbiol. 2009. PMID: 18997032 Free PMC article.
• The helicase-like domains of type III restriction enzymes trigger long-range diffusion along DNA.
  Schwarz FW, Tóth J, van Aelst K, Cui G, Clausing S, Szczelkun MD, Seidel R. Schwarz FW, et al. Science. 2013 Apr 19;340(6130):353-6. doi: 10.1126/science.1231122. Science. 2013. PMID: 23599494 Free PMC article.
• Genome-wide DNA methylation analysis of Haloferax volcanii H26 and identification of DNA methyltransferase related PD-(D/E)XK nuclease family protein HVO_A0006.
  Ouellette M, Jackson L, Chimileski S, Papke RT. Ouellette M, et al. Front Microbiol. 2015 Apr 8;6:251. doi: 10.3389/fmicb.2015.00251. eCollection 2015. Front Microbiol. 2015. PMID: 25904898 Free PMC article.
• Genome of bacteriophage P1.
  Łobocka MB, Rose DJ, Plunkett G 3rd, Rusin M, Samojedny A, Lehnherr H, Yarmolinsky MB, Blattner FR. Łobocka MB, et al. J Bacteriol. 2004 Nov;186(21):7032-68. doi: 10.1128/JB.186.21.7032-7068.2004. J Bacteriol. 2004. PMID: 15489417 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Nature Publishing Group

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • REBASE - The Restriction Enzyme Database