DNA binding activity of recombinant SRY from normal males and XY females - PubMed (original) (raw)

. 1992 Jan 24;255(5043):453-6.

doi: 10.1126/science.1734522.

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DNA binding activity of recombinant SRY from normal males and XY females

V R Harley et al. Science. 1992.

Abstract

The protein encoded by the human testis determining gene, SRY, contains a high mobility group (HMG) box related to that present in the T cell-specific, DNA-binding protein TCF-1. Recombinant SRY protein was able to bind to the same core sequence AACAAAG recognized by TCF-1 in a sequence dependent manner. In five XY females point mutations were found in the region encoding the HMG box. In four cases DNA binding activity of mutant SRY protein was negligible; in the fifth case DNA binding was reduced. These results imply that the DNA binding activity of SRY is required for sex determination.

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