A critical role for the ubiquitin-conjugating enzyme Ubc13 in initiating homologous recombination - PubMed (original) (raw)

. 2007 Mar 9;25(5):663-75.

doi: 10.1016/j.molcel.2007.01.029.

Eiichiro Sonoda, Louise J Barber, Hayato Oka, Yasuhiro Murakawa, Kouichi Yamada, Tsuyoshi Ikura, Xin Wang, Masahiko Kobayashi, Kenichi Yamamoto, Simon J Boulton, Shunichi Takeda

Affiliations

Free article

A critical role for the ubiquitin-conjugating enzyme Ubc13 in initiating homologous recombination

Guang Yu Zhao et al. Mol Cell. 2007.

Free article

Abstract

The ubiquitin (Ub)-conjugating enzyme Ubc13 is implicated in Rad6/Rad18-dependent postreplication repair (PRR) in budding yeast, but its function in vertebrates is not known. We show here that disruption or siRNA depletion of UBC13 in chicken DT40 or human cells confers severe growth defects due to chromosome instability, and hypersensitivity to both UV and ionizing radiation, consistent with a conserved role for Ubc13 in PRR. Remarkably, Ubc13-deficient cells are also compromised for DNA double-strand break (DSB) repair by homologous recombination (HR). Recruitment and activation of the E3 Ub ligase function of BRCA1 and the subsequent formation of the Rad51 nucleoprotein filament at DSBs are abolished in Ubc13-deficient cells. Furthermore, generation of ssDNA/RPA complexes at DSBs is severely attenuated in the absence of Ubc13. These data reveal a critical and unexpected role for vertebrate Ubc13 in the initiation of HR at the level of DSB processing.

PubMed Disclaimer

Similar articles

Cited by

Publication types

MeSH terms

Substances

LinkOut - more resources