Flk1 induced by MSC treatment amplifies angiogenesis and vascular stabilization after stroke - PubMed (original) (raw)

Figure 1

Marrow stromal cell treatment of stroke rats decrease BBB leakage and increase Ang1, Tie2, and occludin expression in the ischemic brain: MCAo rats were treated with or without MSC after stroke. (A, D, G, and J) BBB leakage measured by Evans blue (A), and Ang1 (D), Tie2 (G), and occludin (J) expression in MCAo control rats, respectively. (B, E, H, and K) Evans blue (B), Ang1 (E), Tie2 (H), and occludin (K) expression in the MSC-treated rats, respectively. (C, F, I, and L) Quantitative data of Evans blue level in the ischemic brain (C), and Ang1 (F), Tie2 (I), and occludin (L)-positive cells percentage of area in the ischemic border. Marrow stromal cell treatment stroke animals significant decrease BBB leakage and increase the expression of Ang1, Tie2, and occludin compared with control MCAo animals. Scale bar (E, H, and K)=50_μ_m. (M to R) Double immunostaining of Ang1 with astrocyte (M, Ang1; N, GFAP; and O, Ang1 with GFAP merged); Ang1 with pericyte (P, Ang1; Q, _α_-SMA; R, Ang1 with _α_-SMA merged). (S to X) Double immunostaining Tie2 with pericyte (S, Tie2; T, _α_-SMA; and U, Tie2 with á-SMA merged); Tie2 with brain endothelial cell (V, Tie2;W, vWF; and X, Tie2 with vWF merged), respectively.