Preadipocyte response and impairment of differentiation in an inflammatory environment - PubMed (original) (raw)

. 2007 May 11;356(3):662-7.

doi: 10.1016/j.bbrc.2007.03.053. Epub 2007 Mar 19.

Affiliations

• PMID: 17383612
• DOI: 10.1016/j.bbrc.2007.03.053

Preadipocyte response and impairment of differentiation in an inflammatory environment

Odile Poulain-Godefroy et al. Biochem Biophys Res Commun. 2007.

Abstract

Recent reports suggest the potential role of toll-like receptor 4 (TLR4) in initiation of inflammatory responses and fatty acid-induced insulin resistance. We describe here the synthesis of pro-inflammatory products in 3T3-L1 preadipocyte cell line after stimulation with lipopolysaccharide (LPS), a TLR4 agonist. Expression profiles of mRNA coding for IL6, CCL2, CCL5, CCL11, NOS2, and PTGS2 demonstrated a higher responsiveness to LPS of these transcripts in preadipocytes than in fully differentiated adipocytes, confirming inflammatory features of preadipocytes. IL6, CCL2, CCL5 and CCL11 were secreted in 3T3-L1 supernatants within 4 h after LPS stimulation. In addition, continuous exposure to LPS during adipocyte differentiation impaired this process as was demonstrated by analysis of mRNA profiles of lipogenesis enzymes (FABP4, GPD1, LPL), adipokines (adiponectin, resistin, visfatin, leptin), and of the transcription factor PPARgamma. This suggests that toll-like receptor mediated activation could regulate maintenance of preadipocyte status, and inflammatory environment encountered in inflamed white adipose tissue.

PubMed Disclaimer

Similar articles

• Innate immunity and adipocyte function: ligand-specific activation of multiple Toll-like receptors modulates cytokine, adipokine, and chemokine secretion in adipocytes.
  Kopp A, Buechler C, Neumeier M, Weigert J, Aslanidis C, Schölmerich J, Schäffler A. Kopp A, et al. Obesity (Silver Spring). 2009 Apr;17(4):648-56. doi: 10.1038/oby.2008.607. Epub 2009 Jan 15. Obesity (Silver Spring). 2009. PMID: 19148127
• Activation of Toll-like receptor 4 is associated with insulin resistance in adipocytes.
  Song MJ, Kim KH, Yoon JM, Kim JB. Song MJ, et al. Biochem Biophys Res Commun. 2006 Aug 4;346(3):739-45. doi: 10.1016/j.bbrc.2006.05.170. Epub 2006 Jun 6. Biochem Biophys Res Commun. 2006. PMID: 16781673
• Adipose tissue depots of Holstein cows are immune responsive: inflammatory gene expression in vitro.
  Mukesh M, Bionaz M, Graugnard DE, Drackley JK, Loor JJ. Mukesh M, et al. Domest Anim Endocrinol. 2010 Apr;38(3):168-78. doi: 10.1016/j.domaniend.2009.10.001. Epub 2009 Nov 6. Domest Anim Endocrinol. 2010. PMID: 19914024
• Glycine increases mRNA adiponectin and diminishes pro-inflammatory adipokines expression in 3T3-L1 cells.
  Garcia-Macedo R, Sanchez-Muñoz F, Almanza-Perez JC, Duran-Reyes G, Alarcon-Aguilar F, Cruz M. Garcia-Macedo R, et al. Eur J Pharmacol. 2008 Jun 10;587(1-3):317-21. doi: 10.1016/j.ejphar.2008.03.051. Epub 2008 Apr 8. Eur J Pharmacol. 2008. PMID: 18499099
• The c-Jun N-terminal kinase mediates the induction of oxidative stress and insulin resistance by palmitate and toll-like receptor 2 and 4 ligands in 3T3-L1 adipocytes.
  Davis JE, Gabler NK, Walker-Daniels J, Spurlock ME. Davis JE, et al. Horm Metab Res. 2009 Jul;41(7):523-30. doi: 10.1055/s-0029-1202852. Epub 2009 Mar 10. Horm Metab Res. 2009. PMID: 19277946

Cited by

• Gut microbiota in overweight and obesity: crosstalk with adipose tissue.
  Cani PD, Van Hul M. Cani PD, et al. Nat Rev Gastroenterol Hepatol. 2024 Mar;21(3):164-183. doi: 10.1038/s41575-023-00867-z. Epub 2023 Dec 8. Nat Rev Gastroenterol Hepatol. 2024. PMID: 38066102 Review.
• Increased body mass index is linked to systemic inflammation through altered chromatin co-accessibility in human preadipocytes.
  Garske KM, Kar A, Comenho C, Balliu B, Pan DZ, Bhagat YV, Rosenberg G, Koka A, Das SS, Miao Z, Sinsheimer JS, Kaprio J, Pietiläinen KH, Pajukanta P. Garske KM, et al. Nat Commun. 2023 Jul 14;14(1):4214. doi: 10.1038/s41467-023-39919-y. Nat Commun. 2023. PMID: 37452040 Free PMC article.
• Mechanisms of obesity- and diabetes mellitus-related pancreatic carcinogenesis: a comprehensive and systematic review.
  Ruze R, Song J, Yin X, Chen Y, Xu R, Wang C, Zhao Y. Ruze R, et al. Signal Transduct Target Ther. 2023 Mar 24;8(1):139. doi: 10.1038/s41392-023-01376-w. Signal Transduct Target Ther. 2023. PMID: 36964133 Free PMC article.
• Regulation of CAMP (cathelicidin antimicrobial peptide) expression in adipocytes by TLR 2 and 4.
  Höpfinger A, Karrasch T, Schäffler A, Schmid A. Höpfinger A, et al. Innate Immun. 2021 Feb;27(2):184-191. doi: 10.1177/1753425920988167. Epub 2021 Jan 28. Innate Immun. 2021. PMID: 33509002 Free PMC article.
• Obese Adipose Tissue Secretion Induces Inflammation in Preadipocytes: Role of Toll-Like Receptor-4.
  Renovato-Martins M, Moreira-Nunes C, Atella GC, Barja-Fidalgo C, Moraes JA. Renovato-Martins M, et al. Nutrients. 2020 Sep 16;12(9):2828. doi: 10.3390/nu12092828. Nutrients. 2020. PMID: 32947825 Free PMC article.

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal