Glucose mediates the translocation of NeuroD1 by O-linked glycosylation - PubMed (original) (raw)

Glucose mediates the translocation of NeuroD1 by O-linked glycosylation

Sreenath S Andrali et al. J Biol Chem. 2007.

Abstract

O-Linked GlcNAc modification of nuclear and cytosolic proteins has been shown to regulate the function of many cellular proteins. Increased O-linked glycosylation, observed under chronic hyperglycemia conditions, has been implicated in the pathogenesis of diabetes. However, the exact role of O-GlcNAc modification in regulating glucose homeostasis remains to be established. We report here that the subcellular localization of the pancreatic beta cell-specific transcription factor NeuroD1 is regulated by O-linked glycosylation in the mouse insulinoma cell line MIN6. Under low glucose conditions, NeuroD1 is mainly in the cytosol. However, treatment of MIN6 cells with high glucose results in O-linked GlcNAc modification of NeuroD1 and its subsequent translocation into the nucleus. Consistent with these data, treatment of MIN6 cells with O-(2-acetamido-2-deoxy-d-glucopyranosylidene)-amino N-phenylcarbamate, an inhibitor of O-GlcNAcase, causes Neuro-D1 localization to the nucleus and induction of insulin gene expression even on low glucose. Furthermore, we demonstrate that NeuroD1 interacts with the O-GlcNAc transferase, OGT only at high concentrations of glucose and depletion of OGT by using small interfering RNA oligos interferes with the nuclear localization of NeuroD1 on high glucose. On low glucose NeuroD1 interacts with the O-GlcNAcase and becomes deglycosylated, which is likely to be important for export of Neuro-D1 into cytosol in the presence of low glucose. In summary, the presented data suggest that glucose regulates the subcellular localization of NeuroD1 in pancreatic beta cells via O-linked GlcNAc modification of NeuroD1 by OGT.

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Figures

Figure 1

Figure 1

Exposure to high glucose increases NeuroD1 binding to the insulin promoter by causing the translocation of NeuroD1 from the cytosol into the nucleus. NeuroD1 binding to the insulin I promoter was analyzed by ChIP assay in MIN6 cells treated with either 3 or 30mM glucose using specific NeuroD1 antibodies. The immunoprecipitated DNA was amplified by PCR using primers against the insulin I promoter, separated on a non-denaturing PAGE gel and visualized by staining with ethidium bromide (Panel A). NeuroD1 binding to the insulin I promoter was quantified by real-time PCR and normalized to NeuroD1 binding activity on 3mM glucose (Panel B). Normal IgG was used as a negative control for immunoprecipitation. Panel C: MIN6 cells were transiently transfected with a GFP-NeuroD1 construct and treated with low (3mM D-Glc) or high (30mM D-Glc) D-glucose overnight. GFP-NeuroD1 was visualized by confocal microscopy. Cell nuclei were stained with DAPI. As a control, MIN6 cell were treated with 30mM L-glucose (30mM L-Glc).

Figure 2

Figure 2

Increased flux via the hexosamine biosynthetic pathway regulates the translocation of NeuroD1 into nucleus in MIN6 cells. GFP-NeuroD1 transfected MIN6 cells were treated overnight with 3 mM glucose in the presence or absence of 2 mM glucosamine (GlcN) (Panel A) or 30 mM glucose with or without 20 μM 6-diazo-5-oxo-norleucine (DON) (Panel B) and GFP-NeuroD1 localization was visualized by fluorescence microscopy. Nuclei were stained with DAPI.

Figure 3

Figure 3

Inhibition of O-GlcNAcase by treatment with PUGNAc on low glucose causes NeuroD1 to localize to the nucleus and to bind to the insulin promoter. After transfection with the GFP-NeuroD1 construct, MIN6 cells were incubated with 3 mM glucose in the presence or absence of 100 μM PUGNAc and the localization of NeuroD1 was visualized by immunoflourescence microscopy (Panel A). Nuclear fractions obtained from MIN6 cells after treatment with 30 or 3 mM glucose with or without PUGNAc (100 μM) were resolved on 10% SDS-PAGE gel and immunoblotted with NeuroD1, histone H3 (nuclear marker) and thiolase (cytosolic marker) antibodies (Panel B). Panel C: ChIP analysis of NeuroD1 binding to the insulin I promoter was carried out in MIN6 cells treated with 30 or 3 mM glucose with or without PUGNAc (100 μM) using NeuroD1 antibodies or normal IgG (as negative control) for immunoprecipitation. The DNA extracted from the immunoprecipitates was used as template for PCR to amplify the insulin I gene promoter. 0.5% of the total DNA was used as input.

Figure 4

Figure 4

High glucose mediates the O-GlcNAc modification of NeuroD1 in MIN6 cells. Protein extracts from MIN6 cells incubated with 30 or 3 mM glucose with or without 100 μM PUGNAc were used for immunoprecipitation with the NeuroD1 specific antibody (IP-NeuroD1). Immunoprecipitates were resolved on 10% SDS-PAGE and immunoblotted with either CTD 110.6 (panel A) or RL2 (panel B) antibodies which specifically recognize O-GlcNAc modification on proteins. Normal IgG was used as negative control. The amount of NeuroD1 immunoprecipitated under various conditions as determined using the NeuroD1 antibodies is similar. Input represents 10% of the total cell lysate used for immunoprecipitation.

Figure 5

Figure 5

NeuroD1 interacts with OGT on high glucose and with O-GlcNAcase on low glucose incubated MIN6 cells. After incubation of MIN6 cells with 3 or 30 mM glucose, protein extracts were prepared and used for immunoprecipitation assays with OGT (panel A) or O-GlcNAcase (panel B) antibodies. Immunoprecipitates were resolved on 10% SDS-PAGE and immunoblotted with NeuroD1 antibodies. The amount of OGT (panel A, IB:OGT) and O-GlcNAcase (panel B, IB:O-GlcNAcase) protein immunoprecipitated from 3 or 30mM glucose incubated cell extracts was similar. Normal IgG was used as a negative control and 10% of the cell lysate used for immunoprecipitation was loaded as input.

Figure 6

Figure 6

Silencing of OGT expression using siRNA oligos, blocks the nuclear localization of NeuroD1 on high glucose. MIN6 cells were co-transfected with the GFP-NeuroD1 construct and siRNA oligos against OGT (si-OGT) or as a negative control with random siRNA oligos (si-Random). OGT protein was detected in cells by incubation with an OGT antibody and a secondary antibody conjugated to Alexa Fluor 594. OGT staining and GFP-NeuroD1 was visualized by confocal microscopy. Nuclei were stained with DAPI.

Figure 7

Figure 7

Treatment with PUGNAc in the presence of low glucose increases insulin gene transcription. MIN6 cells incubated with 30mM or 3mM glucose with or without 100μM PUGNAc were used for isolation of total RNA. The levels of insulin I and beta-actin mRNA were quantified using real-time RT-PCR in three independent experiments (n=3). The graph represents the fold changes in insulin I mRNA levels compared to 3mM glucose incubated MIN6 cells and is normalized for beta-actin levels. * p <0.01 and ** p<0.03.

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