Genetic reconstruction of a functional transcriptional regulatory network - PubMed (original) (raw)
Comparative Study
doi: 10.1038/ng2012. Epub 2007 Apr 8.
Affiliations
- PMID: 17417638
- DOI: 10.1038/ng2012
Comparative Study
Genetic reconstruction of a functional transcriptional regulatory network
Zhanzhi Hu et al. Nat Genet. 2007 May.
Abstract
Although global analyses of transcription factor binding provide one view of potential transcriptional regulatory networks, regulation also occurs at levels distinct from transcription factor binding. Here, we use a genetic approach to identify targets of transcription factors in yeast and reconstruct a functional regulatory network. First, we profiled transcriptional responses in S. cerevisiae strains with individual deletions of 263 transcription factors. Then we used directed-weighted graph modeling and regulatory epistasis analysis to identify indirect regulatory relationships between these transcription factors, and from this we reconstructed a functional transcriptional regulatory network. The enrichment of promoter motifs and Gene Ontology annotations provide insight into the biological functions of the transcription factors.
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