Single particle tracking. Analysis of diffusion and flow in two-dimensional systems - PubMed (original) (raw)
Single particle tracking. Analysis of diffusion and flow in two-dimensional systems
H Qian et al. Biophys J. 1991 Oct.
Abstract
Analysis of the trajectories of small particles at high spatial and temporal resolution using video enhanced contrast microscopy provides a powerful approach to characterizing the mechanisms of particle motion in living cells and in other systems. We present here the theoretical basis for the analysis of these trajectories for particles undergoing random diffusion and/or systematic transport at uniform velocity in two-dimensional systems. The single particle tracking method, based on observations of the trajectories of individual particles, is compared with methods that characterize the motions of a large collection of particles such as fluorescence photobleaching recovery. Determination of diffusion coefficients or transport velocities either from correlation of positions or of velocities of the particles is discussed. A result of practical importance is an analysis of the dependence of the expected statistical uncertainty of these determinations on the number of position measurements. This provides a way of judging the accuracy of the diffusion coefficients and transport velocities obtained using this approach.
Similar articles
- Single-particle tracking: models of directed transport.
Saxton MJ. Saxton MJ. Biophys J. 1994 Nov;67(5):2110-9. doi: 10.1016/S0006-3495(94)80694-0. Biophys J. 1994. PMID: 7858148 Free PMC article. - How to quantify protein diffusion in the bacterial membrane.
van den Wildenberg SM, Bollen YJ, Peterman EJ. van den Wildenberg SM, et al. Biopolymers. 2011 May;95(5):312-21. doi: 10.1002/bip.21585. Epub 2011 Jan 14. Biopolymers. 2011. PMID: 21240922 Review. - Mobility measurement by analysis of fluorescence photobleaching recovery kinetics.
Axelrod D, Koppel DE, Schlessinger J, Elson E, Webb WW. Axelrod D, et al. Biophys J. 1976 Sep;16(9):1055-69. doi: 10.1016/S0006-3495(76)85755-4. Biophys J. 1976. PMID: 786399 Free PMC article. - Single-particle tracking: applications to membrane dynamics.
Saxton MJ, Jacobson K. Saxton MJ, et al. Annu Rev Biophys Biomol Struct. 1997;26:373-99. doi: 10.1146/annurev.biophys.26.1.373. Annu Rev Biophys Biomol Struct. 1997. PMID: 9241424 Review.
Cited by
- Diffusion of individual nanoparticles in cylindrical diatom frustule.
Tomioka N, Abe Y, Matsuda Y. Tomioka N, et al. Nanoscale Adv. 2024 Oct 8. doi: 10.1039/d4na00576g. Online ahead of print. Nanoscale Adv. 2024. PMID: 39391628 Free PMC article. - Self-inspired learning for denoising live-cell super-resolution microscopy.
Qu L, Zhao S, Huang Y, Ye X, Wang K, Liu Y, Liu X, Mao H, Hu G, Chen W, Guo C, He J, Tan J, Li H, Chen L, Zhao W. Qu L, et al. Nat Methods. 2024 Oct;21(10):1895-1908. doi: 10.1038/s41592-024-02400-9. Epub 2024 Sep 11. Nat Methods. 2024. PMID: 39261639 - Quantifying surface tension and viscosity in biomolecular condensates by FRAP-ID.
Santamaria A, Hutin S, Doucet CM, Zubieta C, Milhiet PE, Costa L. Santamaria A, et al. Biophys J. 2024 Oct 1;123(19):3366-3374. doi: 10.1016/j.bpj.2024.07.043. Epub 2024 Aug 8. Biophys J. 2024. PMID: 39113361 - Overstretching alveolar epithelial type II cells decreases surfactant secretion via actin polymerization and intracellular trafficking alteration.
Inoue S, Nagao J, Kawamoto K, Kan-O K, Fukuyama S, Sasaki S, Kudo S, Okamoto I, Sera T. Inoue S, et al. Heliyon. 2024 Jun 22;10(13):e33499. doi: 10.1016/j.heliyon.2024.e33499. eCollection 2024 Jul 15. Heliyon. 2024. PMID: 39040228 Free PMC article. - Annihilation dynamics during spiral defect chaos revealed by particle models.
Tyree TJ, Murphy P, Rappel WJ. Tyree TJ, et al. Chaos. 2024 May 1;34(5):053131. doi: 10.1063/5.0203319. Chaos. 2024. PMID: 38787314
References
- J Cell Biol. 1986 Jan;102(1):88-96 - PubMed
- Cell Motil Cytoskeleton. 1988;9(1):30-47 - PubMed
- Science. 1988 Jan 1;239(4835):61-4 - PubMed
- Nature. 1988 Feb 4;331(6155):450-3 - PubMed
- Nature. 1989 Jul 27;340(6231):315-7 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources