Mouse neutrophilic granulocytes express mRNA encoding the macrophage colony-stimulating factor receptor (CSF-1R) as well as many other macrophage-specific transcripts and can transdifferentiate into macrophages in vitro in response to CSF-1 - PubMed (original) (raw)

doi: 10.1189/jlb.1206713. Epub 2007 Apr 16.

Affiliations

PMID: 17438263
DOI: 10.1189/jlb.1206713

Mouse neutrophilic granulocytes express mRNA encoding the macrophage colony-stimulating factor receptor (CSF-1R) as well as many other macrophage-specific transcripts and can transdifferentiate into macrophages in vitro in response to CSF-1

R Tedjo Sasmono et al. J Leukoc Biol. 2007 Jul.

Abstract

The differentiation of macrophages from their progenitors is controlled by macrophage colony-stimulating factor (CSF-1), which binds to a receptor (CSF-1R) encoded by the c-fms proto-oncogene. We have previously used the promoter region of the CSF-1R gene to direct expression of an enhanced green fluorescent protein (EGFP) reporter gene to resident macrophage populations in transgenic mice. In this paper, we show that the EGFP reporter is also expressed in all granulocytes detected with the Gr-1 antibody, which binds to Ly-6C and Ly-6G or with a Ly-6G-specific antibody. Transgene expression reflects the presence of CSF-1R mRNA but not CSF-1R protein. The same pattern is observed with the macrophage-specific F4/80 marker. Based on these findings, we performed a comparative array profiling of highly purified granulocytes and macrophages. The patterns of mRNA expression differed predominantly through granulocyte-specific expression of a small subset of transcription factors (Egr1, HoxB7, STAT3), known abundant granulocyte proteins (e.g., S100A8, S100A9, neutrophil elastase), and specific receptors (fMLP, G-CSF). These findings suggested that appropriate stimuli might mediate rapid interconversion of the major myeloid cell types, for example, in inflammation. In keeping with this hypothesis, we showed that purified Ly-6G-positive granulocytes express CSF-1R after overnight culture and can subsequently differentiate to form F4/80-positive macrophages in response to CSF-1.

PubMed Disclaimer

Cited by

Absence of Cytotoxicity towards Microglia of Iron Oxide (α-Fe2O3) Nanorhombohedra.
Lewis CS, Torres L, Miyauchi JT, Rastegar C, Patete JM, Smith JM, Wong SS, Tsirka SE. Lewis CS, et al. Toxicol Res (Camb). 2016 May 1;5(3):836-847. doi: 10.1039/C5TX00421G. Epub 2016 Feb 26. Toxicol Res (Camb). 2016. PMID: 27274811 Free PMC article.
Neutrophil apoptosis: relevance to the innate immune response and inflammatory disease.
Fox S, Leitch AE, Duffin R, Haslett C, Rossi AG. Fox S, et al. J Innate Immun. 2010;2(3):216-27. doi: 10.1159/000284367. Epub 2010 Feb 11. J Innate Immun. 2010. PMID: 20375550 Free PMC article. Review.
Relevance of granulocyte apoptosis to resolution of inflammation at the respiratory mucosa.
Leitch AE, Duffin R, Haslett C, Rossi AG. Leitch AE, et al. Mucosal Immunol. 2008 Sep;1(5):350-63. doi: 10.1038/mi.2008.31. Epub 2008 Jul 2. Mucosal Immunol. 2008. PMID: 19079199 Free PMC article. Review.
Tannic acid-modified silver nanoparticles for wound healing: the importance of size.
Orlowski P, Zmigrodzka M, Tomaszewska E, Ranoszek-Soliwoda K, Czupryn M, Antos-Bielska M, Szemraj J, Celichowski G, Grobelny J, Krzyzowska M. Orlowski P, et al. Int J Nanomedicine. 2018 Feb 16;13:991-1007. doi: 10.2147/IJN.S154797. eCollection 2018. Int J Nanomedicine. 2018. PMID: 29497293 Free PMC article.
Cre-ativity in the liver: transgenic approaches to targeting hepatic nonparenchymal cells.
Greenhalgh SN, Conroy KP, Henderson NC. Greenhalgh SN, et al. Hepatology. 2015 Jun;61(6):2091-9. doi: 10.1002/hep.27606. Epub 2015 Apr 13. Hepatology. 2015. PMID: 25412828 Free PMC article. Review.

Mouse neutrophilic granulocytes express mRNA encoding the macrophage colony-stimulating factor receptor (CSF-1R) as well as many other macrophage-specific transcripts and can transdifferentiate into macrophages in vitro in response to CSF-1 - PubMed (original) (raw)