Identification of a gene cluster enabling Lactobacillus casei BL23 to utilize myo-inositol - PubMed (original) (raw)

Proposed MI catabolic pathway in L. casei BL23. For the B. subtilis iol genes, the functions of only iolT, iolG, and iolE have been experimentally elucidated. The functions of the other genes are assigned based on sequence homology, and their gene products are underlined. The function of iolB is unknown, but in Legionella pneumophila, iolB is fused to iolC (accession number YP_123940), which is probably coding for a 2-deoxy-5-keto-

d

-gluconate kinase. In L. casei, iolK (encoding a probable malonic semialdehyde decarboxylase) might define an alternative pathway for the conversion of malonic semialdehyde to acetaldehyde. The metabolites are numbered as follows: (1), MI; (2), inosose; (3),

d

-2,3-diketo-4-deoxy-epi-inositol; (4), 2-deoxy-5-keto-

d

-gluconate; (5), 2-deoxy-5-keto-

d

-gluconate-6-phosphate; (6), dihydroxyacetone-phosphate; (7), malonic semialdehyde; (8) acetyl-CoA; (9), acetaldehyde.