Monocyte chemoattractant protein-1 in subcutaneous abdominal adipose tissue: characterization of interstitial concentration and regulation of gene expression by insulin - PubMed (original) (raw)
. 2007 Jul;92(7):2688-95.
doi: 10.1210/jc.2006-2814. Epub 2007 Apr 24.
Affiliations
- PMID: 17456576
- DOI: 10.1210/jc.2006-2814
Monocyte chemoattractant protein-1 in subcutaneous abdominal adipose tissue: characterization of interstitial concentration and regulation of gene expression by insulin
Giuseppe Murdolo et al. J Clin Endocrinol Metab. 2007 Jul.
Abstract
Context: The chemokine monocyte chemoattractant protein-1 (MCP-1) is implicated in obesity-associated chronic inflammation, insulin resistance, and atherosclerosis.
Objectives: The objectives of this study were to: 1) characterize the interstitial levels and the gene expression of MCP-1 in the sc abdominal adipose tissue (SCAAT), 2) elucidate the response of MCP-1 to acute hyperinsulinemia, and 3) determine the relationship between MCP-1 and arterial stiffness.
Design: Nine lean (L) and nine uncomplicated obese (OB) males were studied in the fasting state and during a euglycemic-hyperinsulinemic clamp combined with the microdialysis technique. Interstitial and serum MCP-1 (iMCP-1 and sMCP-1, respectively) levels, pulse wave analysis, and SCAAT biopsies were characterized at baseline and after hyperinsulinemia.
Results: OB showed elevated sMCP-1 (P < 0.01) but similar iMCP-1 levels as compared with L. Basal iMCP-1 concentrations were considerably higher than sMCP-1 (P < 0.0001), and a gradient between iMCP-1 and sMCP-1 levels was maintained throughout the hyperinsulinemia. At baseline, SCAAT gene expression profile revealed a "co-upregulation" of MCP-1, MCP-2, macrophage inflammatory protein-1alpha, and CD68 in OB, and whole-body glucose disposal inversely correlated with the MCP-1 gene expression. After hyperinsulinemia, MCP-1 and MCP-2 mRNA levels significantly increased in L, but not in OB. Finally, sMCP-1 excess in the OB positively correlated with the stiffer vasculature.
Conclusions: These observations demonstrate similar interstitial concentrations and a differential gene response to hyperinsulinemia of MCP-1 in the SCAAT from L and OB individuals. In human obesity, we suggest the SCAAT MCP-1 gene overexpression as a biomarker of an "inflamed" adipose organ and impaired glucose metabolism.
Similar articles
- Insulin regulation of MCP-1 in human adipose tissue of obese and lean women.
Westerbacka J, Cornér A, Kolak M, Makkonen J, Turpeinen U, Hamsten A, Fisher RM, Yki-Järvinen H. Westerbacka J, et al. Am J Physiol Endocrinol Metab. 2008 May;294(5):E841-5. doi: 10.1152/ajpendo.00653.2006. Epub 2008 Feb 12. Am J Physiol Endocrinol Metab. 2008. PMID: 18270300 - Insulin differentially modulates the peripheral endocannabinoid system in human subcutaneous abdominal adipose tissue from lean and obese individuals.
Murdolo G, Kempf K, Hammarstedt A, Herder C, Smith U, Jansson PA. Murdolo G, et al. J Endocrinol Invest. 2007 Sep;30(8):RC17-21. doi: 10.1007/BF03347440. J Endocrinol Invest. 2007. PMID: 17923791 Clinical Trial. - Effect of hyperinsulinemia and very-low-calorie diet on interstitial cytokine levels in subcutaneous adipose tissue of obese women.
Siklova-Vitkova M, Polak J, Klimcakova E, Vrzalova J, Hejnova J, Kovacikova M, Kovacova Z, Bajzova M, Rossmeislova L, Hnevkovska Z, Langin D, Stich V. Siklova-Vitkova M, et al. Am J Physiol Endocrinol Metab. 2009 Nov;297(5):E1154-61. doi: 10.1152/ajpendo.00086.2009. Epub 2009 Sep 1. Am J Physiol Endocrinol Metab. 2009. PMID: 19724021 - Multihormonal control of ob gene expression and leptin secretion from cultured human visceral adipose tissue: increased responsiveness to glucocorticoids in obesity.
Halleux CM, Servais I, Reul BA, Detry R, Brichard SM. Halleux CM, et al. J Clin Endocrinol Metab. 1998 Mar;83(3):902-10. doi: 10.1210/jcem.83.3.4644. J Clin Endocrinol Metab. 1998. PMID: 9506746 Review. - Insulin resistance and hyperinsulinemia: is hyperinsulinemia the cart or the horse?
Shanik MH, Xu Y, Skrha J, Dankner R, Zick Y, Roth J. Shanik MH, et al. Diabetes Care. 2008 Feb;31 Suppl 2:S262-8. doi: 10.2337/dc08-s264. Diabetes Care. 2008. PMID: 18227495 Review.
Cited by
- Metabolic dysfunction in obesity is related to impaired suppression of fatty acid release from adipose tissue by insulin.
Schleh MW, Ryan BJ, Ahn C, Ludzki AC, Varshney P, Gillen JB, Van Pelt DW, Pitchford LM, Howton SM, Rode T, Chenevert TL, Hummel SL, Burant CF, Horowitz JF. Schleh MW, et al. Obesity (Silver Spring). 2023 May;31(5):1347-1361. doi: 10.1002/oby.23734. Epub 2023 Mar 29. Obesity (Silver Spring). 2023. PMID: 36988872 Free PMC article. - Fat Cell Size: Measurement Methods, Pathophysiological Origins, and Relationships With Metabolic Dysregulations.
Ye RZ, Richard G, Gévry N, Tchernof A, Carpentier AC. Ye RZ, et al. Endocr Rev. 2022 Jan 12;43(1):35-60. doi: 10.1210/endrev/bnab018. Endocr Rev. 2022. PMID: 34100954 Free PMC article. - Factors Affecting Metabolic Outcomes Post Bariatric Surgery: Role of Adipose Tissue.
Keshavjee SH, Schwenger KJP, Yadav J, Jackson TD, Okrainec A, Allard JP. Keshavjee SH, et al. J Clin Med. 2021 Feb 11;10(4):714. doi: 10.3390/jcm10040714. J Clin Med. 2021. PMID: 33670215 Free PMC article. Review. - Dietary polyunsaturated fatty acids modulate adipose secretome and is associated with changes in mammary epithelial stem cell self-renewal.
Hill EM, Esper RM, Sen A, Simon BR, Aslam MN, Jiang Y, Dame MK, McClintock SD, Colacino JA, Djuric Z, Wicha MS, Smith WL, Brenner DE. Hill EM, et al. J Nutr Biochem. 2019 Sep;71:45-53. doi: 10.1016/j.jnutbio.2019.05.007. Epub 2019 May 24. J Nutr Biochem. 2019. PMID: 31272031 Free PMC article. - Intraoperative immunomodulatory effects of sevoflurane versus total intravenous anesthesia with propofol in bariatric surgery (the OBESITA trial): study protocol for a randomized controlled pilot trial.
de Sousa GC, Cruz FF, Heil LB, Sobrinho CJS, Saddy F, Knibel FP, Pereira JB, Schultz MJ, Pelosi P, Gama de Abreu M, Silva PL, Rocco PRM. de Sousa GC, et al. Trials. 2019 May 28;20(1):300. doi: 10.1186/s13063-019-3399-z. Trials. 2019. PMID: 31138279 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Research Materials
Miscellaneous