Sweet taste receptors in rat small intestine stimulate glucose absorption through apical GLUT2 - PubMed (original) (raw)

A, effect on 20 m

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glucose absorption of sucralose (▴, 1 m

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, S arrowhead) added at 30 min and of phloretin added at 40 min (▪, 1 m

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P arrowhead): effect on sucralose-stimulated glucose absorption (0–40 min) of phloretin added at 40 min (▪). B, concentration dependence of stimulation of glucose absorption by sucralose; 20 m

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glucose and sucralose were perfused in the absence (formula image, 0–40 min) and presence (formula image, 40–80 min) of 1 m

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phloretin. C_–_E, role of PLC β2 for (C) 20 m

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glucose (C), (D) 20 m

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glucose + 1 m

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sucralose (C) and (E) 75 m

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glucose (C); open bar perfusion for 0–40 min versus hatched bar for 40–80 min; U, 10 μ

m

U-73122; P, 1 m

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phloretin. F, comparison of effects of sucralose (S), acesulfame potassium (K) and saccharin (Sa) on the components of 20 m

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glucose absorption (C) determined after addition of phloretin (P) at 40 min. Absorption rate in μmol min−1 (g dry weight)−1; values are means ±

s.e.m

, _n_= 4–6 for each condition. Student's t test: unpaired comparison for different perfusions, open bar for experiment with open bar for control (C only): *P < 0.05, **P < 0.01, ***P < 0.001, paired comparison within the same perfusion; hatched bar with adjoining open bar, +P < 0.05, ++P < 0.01, +++P < 0.001.