Hydrogen peroxide triggered prochelator activation, subsequent metal chelation, and attenuation of the fenton reaction - PubMed (original) (raw)

Effect of SIH and SIH-B on the oxidative degradation of 2-deoxyribose promoted by a) FeIII or b) CuII in the presence of H2O2 and hydroascorbate. SIH-B (50 µ

m

) was preincubated with H2O2 for 45 min at 298 K, then the solution (0.5 mL) was added to the assay system containing FeIII (or CuII; 25 µ

m

), 2-deoxyribose (10 m

m

), and hydroascorbate (200 µ

m

) in KPB (20 m

m

, pH 7.2). c) Effect of preincubation time (0, 5, 10, 20, 40 min) and [SIH-B]/[Fe] ratio (r) on attenuation of FeII-promoted Fenton chemistry in KPB buffer (20 m

m

, pH 7.2). [Fe] = 10 µ

m

, [H2O2] = 200 µ

m

; A and _A_0 are the absorbance at 532 nm in the presence and absence of SIH-B, respectively.