Phosphoinositide metabolism and insulin secretion from isolated rat pancreatic islets - PubMed (original) (raw)
- PMID: 175090
- PMCID: PMC436702
- DOI: 10.1172/JCI108325
Phosphoinositide metabolism and insulin secretion from isolated rat pancreatic islets
R S Clements Jr et al. J Clin Invest. 1976 Mar.
Abstract
Since many cell types have been shown to respond to extracellular stimulation with a rapid increase in phosphatidylinositol turnover, the present studies were undertaken to determine whether carbohydrate-stimulated insulin secretion from the isolated rat pancreatic islet is accompanied by detectable alterations in the phosphatidylinositol metabolism of this tissue. We have demonstrated that rat pancreatic islets incubated with tritiated myo-inositol rapidly incorporate radioactivity into islet phosphatidylinositol. Incubation of prelabeled islets with elevated concentrations of carbohydrates which stimulate insulin secretion (D-glucose and D-mannose) results in a decrease in the recovery of lipid-bound radioactivity, whereas incubation with carbohydrates which do not stimulate insulin secretion (D-galactose and myo-inositol) has no effect upon the recovery of lipid-bound radioactivity. Within 2 min of exposure of prelabeled islets to elevated concentrations of D-glucose, a decrease in the recovery of [2-3H]myo-inositol-derived radioactivity in islet phosphatidylinositol can be demonstrated. When islets prelabeled with [2-3H]myo-inositol are perifused with elevated concentrations of D-glucose or D-mannose (but not D-galactose or myoinositol) a rapid and transient increase in the rate of extracellular release of water-soluble radioactivity is observed. Since a significant fraction of the radioactivity released under these conditions is in the form of myo-inositol phosphate, cyclic myo-inositol-1,2-phosphate, and glycerophosphorylmyo-inositol, it is presumably derived from the cleavage of labeled islet phosphatidylinositol. It is speculated that alterations in the metabolism of myo-inositol-containing phospholipids may have a functional role in the process of insulin secretion from the pancreatic beta cell.
Similar articles
- Substrate requirements for the phosphoinositide response in rat pancreatic islets.
Clements RS Jr, Evans MH, Pace CS. Clements RS Jr, et al. Biochim Biophys Acta. 1981 Apr 17;674(1):1-9. doi: 10.1016/0304-4165(81)90340-8. Biochim Biophys Acta. 1981. PMID: 6263344 - Subcellular localization of the alterations in phosphatidylinositol metabolism following glucose-induced insulin release from rat pancreatic islets.
Clements RS Jr, Rhoten WB, Starnes WR. Clements RS Jr, et al. Diabetes. 1977 Dec;26(12):1109-16. doi: 10.2337/diab.26.12.1109. Diabetes. 1977. PMID: 338403 - Rapid transient efflux of phosphate ions from pancreatic islets as an early action of insulin secretagogues.
Freinkel N, Younsi CE, Bonnar J, Dawson RM. Freinkel N, et al. J Clin Invest. 1974 Nov;54(5):1179-89. doi: 10.1172/JCI107861. J Clin Invest. 1974. PMID: 4607816 Free PMC article. - Metabolism of glucose and mannose anomers in pancreatic islets.
Malaisse WJ, Malaisse-Lagae F, Sener A. Malaisse WJ, et al. Reprod Nutr Dev (1980). 1983;23(2 B):321-7. doi: 10.1051/rnd:19830301. Reprod Nutr Dev (1980). 1983. PMID: 6223355 Review. - Phosphoinositide metabolism and insulin secretion.
Ilkova HM. Ilkova HM. Diabete Metab. 1994 Mar-Apr;20(2):150-6. Diabete Metab. 1994. PMID: 7805952 Review.
Cited by
- The conditions under which rat islets are labelled with [3H]inositol alter the subsequent responses of these islets to a high glucose concentration.
Zawalich WS, Zawalich KC, Rasmussen H. Zawalich WS, et al. Biochem J. 1989 May 1;259(3):743-9. doi: 10.1042/bj2590743. Biochem J. 1989. PMID: 2658979 Free PMC article. - ADP-ribosylation factor 6 regulates insulin secretion through plasma membrane phosphatidylinositol 4,5-bisphosphate.
Lawrence JT, Birnbaum MJ. Lawrence JT, et al. Proc Natl Acad Sci U S A. 2003 Nov 11;100(23):13320-5. doi: 10.1073/pnas.2232129100. Epub 2003 Oct 29. Proc Natl Acad Sci U S A. 2003. PMID: 14585928 Free PMC article. - GTP- and inositol 1,4,5-trisphosphate-induced release of 45Ca2+ from a membrane store co-localized with pancreatic-islet-cell plasma membrane.
Dunlop ME, Larkins RG. Dunlop ME, et al. Biochem J. 1988 Jul 1;253(1):67-72. doi: 10.1042/bj2530067. Biochem J. 1988. PMID: 2458719 Free PMC article. - Role of Ca2+ in secretagogue-stimulated breakdown of phosphatidylinositol in rat pancreatic islets.
Axen KV, Schubart UK, Blake AD, Fleischer N. Axen KV, et al. J Clin Invest. 1983 Jul;72(1):13-21. doi: 10.1172/jci110951. J Clin Invest. 1983. PMID: 6192142 Free PMC article. - Relationship between hormonal activation of phosphatidylinositol hydrolysis, fluid secretion and calcium flux in the blowfly salivary gland.
Fain JN, Berridge MJ. Fain JN, et al. Biochem J. 1979 Jan 15;178(1):45-58. doi: 10.1042/bj1780045. Biochem J. 1979. PMID: 219851 Free PMC article.
References
- Biochim Biophys Acta. 1975 Mar 25;415(1):81-47 - PubMed
- Diabetologia. 1973 Jun;9(3):167-73 - PubMed
- Biochem J. 1975 Jan;146(1):247-52 - PubMed
- Biochem Biophys Res Commun. 1971 Sep;44(5):1258-64 - PubMed
- J Biol Chem. 1964 May;239:1369-73 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources